高级检索

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

不同质量浓度NAA、IBA对栓皮栎、蒙古栎黄化嫩枝扦插生根的影响

张杰 李健康 段安安 孙宇涵 刘洪山 武素然 李贵芬 李云

张杰, 李健康, 段安安, 孙宇涵, 刘洪山, 武素然, 李贵芬, 李云. 不同质量浓度NAA、IBA对栓皮栎、蒙古栎黄化嫩枝扦插生根的影响[J]. 北京林业大学学报, 2019, 41(7): 128-138. doi: 10.13332/j.1000-1522.20190103
引用本文: 张杰, 李健康, 段安安, 孙宇涵, 刘洪山, 武素然, 李贵芬, 李云. 不同质量浓度NAA、IBA对栓皮栎、蒙古栎黄化嫩枝扦插生根的影响[J]. 北京林业大学学报, 2019, 41(7): 128-138. doi: 10.13332/j.1000-1522.20190103
Zhang Jie, Li Jiankang, Duan An’an, Sun Yuhan, Liu Hongshan, Wu Suran, Li Guifen, Li Yun. Effects of different concentrations of IBA, NAA on rooting of etiolated shoots of Quercus variabilis and Quercus mongolica[J]. Journal of Beijing Forestry University, 2019, 41(7): 128-138. doi: 10.13332/j.1000-1522.20190103
Citation: Zhang Jie, Li Jiankang, Duan An’an, Sun Yuhan, Liu Hongshan, Wu Suran, Li Guifen, Li Yun. Effects of different concentrations of IBA, NAA on rooting of etiolated shoots of Quercus variabilis and Quercus mongolica[J]. Journal of Beijing Forestry University, 2019, 41(7): 128-138. doi: 10.13332/j.1000-1522.20190103

不同质量浓度NAA、IBA对栓皮栎、蒙古栎黄化嫩枝扦插生根的影响

doi: 10.13332/j.1000-1522.20190103
基金项目: 北京林业大学2019年度一流学科建设项目(2019XKJS0308),北京林业大学重大科研成果培育项目(2017CGP007),中央高校基本科研业务费专项(2016BLPX13)
详细信息
    作者简介:

    张杰。主要研究方向:林木遗传育种。Email:872404121@qq.com 地址:650224云南省昆明市盘龙区白龙寺300号西南林业大学林学院

    责任作者:

    孙宇涵,副教授。主要研究方向:林木遗传育种和生物技术。Email:syh831008@163.com 地址:同上

    李云,教授,博士生导师。主要研究方向:林木遗传育种和生物技术。Email:yunli@bjfu.du.cn 地址:100083北京市海淀区清华东路35号北京林业大学生物科学与技术学院

  • 中图分类号: S722.8

Effects of different concentrations of IBA, NAA on rooting of etiolated shoots of Quercus variabilis and Quercus mongolica

  • 摘要: 目的以不同质量浓度的NAA、IBA对选育的栓皮栎、蒙古栎优良种源当年生实生苗半木质化黄化嫩枝扦插生根的影响,探究何种质量浓度下最有利于其扦插生根,以期选出最合适的质量浓度组合,为今后栓皮栎和蒙古栎的无性选育提供技术支持。方法本研究以选育的栓皮栎、蒙古栎优良种源当年生实生苗半木质化黄化嫩枝为材料,采用萘乙酸(NAA)、吲哚丁酸(IBA)2种植物生长调节剂,分别设置6个质量浓度(3 000、2 000、1 500、750、600、400 mg/L),并用清水、未黄化实生苗半木质化嫩枝为对照,调查不同质量浓度NAA、IBA对栓皮栎、蒙古栎黄化嫩枝扦插生根的影响。结果黄化插穗在生根率、生根数、根长和根径方面均明显优于未黄化插穗,2种植物生长调节剂对黄化栓皮栎、蒙古栎嫩枝插穗不定根诱导差异显著。IBA分别为2 000、1 500、750、600、400 mg/L时处理黄化或未黄化栓皮栎、蒙古栎嫩枝的生根效果均优于NAA相对应的5个不同质量浓度处理的生根效果。在生长调节剂各个质量浓度条件下,处理的黄化栓皮栎、蒙古栎插穗,其不定根发生率、生根数等生根指标均明显高于未黄化插穗所对应的指标。当质量浓度为1 500 mg/L时,栓皮栎黄化嫩枝生根率比未黄化栓皮栎嫩枝的还要高20.0%,蒙古栎黄化嫩枝生根率比未黄化蒙古栎嫩枝的还要高14.6%。结论栓皮栎、蒙古栎优良种源当年生实生苗半木质化的黄化与未黄化嫩枝扦插繁殖的最佳植物生长调节剂处理剂量均为1 500 mg/L的IBA。其不定根发生率、生根数等生根指标均最大,其中黄化栓皮栎插穗生根率达100%,黄化蒙古栎插穗生根率达84.6%。

     

  • 图  1  不同质量浓度NAA对栓皮栎黄化嫩枝扦插生根的影响

    Figure  1.  Effects of different mass concentrations of NAA on the rooting of etiolated Quercus variabilis shoots

    图  2  不同质量浓度NAA对栓皮栎未黄化嫩枝扦插生根的影响

    Figure  2.  Effects of different mass concentrations of NAA on the rooting of non-etiolated Quercus variabilis shoots

    图  3  不同质量浓度IBA对栓皮栎黄化嫩枝扦插生根的影响

    Figure  3.  Effects of different mass concentrations of IBA on the rooting of etiolated Quercus variabilis shoots

    图  4  不同质量浓度IBA对栓皮栎未黄化嫩枝扦插生根的影响

    Figure  4.  Effects of different mass concentrations of IBA on the rooting of non-etiolated Quercus variabilis shoots

    图  5  不同质量浓度NAA对蒙古栎黄化嫩枝扦插生根的影响

    Figure  5.  Effects of different mass concentrations of NAA on the rooting of etiolated Quercus mongolica shoots

    图  6  不同质量浓度NAA对蒙古栎未黄化嫩枝扦插生根的影响

    Figure  6.  Effects of different mass concentrations of NAA on the rooting of non-etiolated Quercus mongolica shoots

    图  7  不同质量浓度IBA对蒙古栎黄化嫩枝扦插生根的影响

    Figure  7.  Effects of different mass concentrations of IBA on the rooting of etiolated Quercus mongolica shoots

    图  8  不同质量浓度IBA对蒙古栎未黄化嫩枝扦插生根的影响

    Figure  8.  Effects of different mass concentrations of IBA on the rooting of non-etiolated Quercus mongolica shoots

    表  1  不同质量浓度NAA对栓皮栎黄化和未黄化嫩枝扦插生根的影响

    Table  1.   Effects of different mass concentrations of NAA on the rooting of etiolated and non-etiolated shoots of Quercus variabilis

    处理条件
    Treatment
    condition
    黄化嫩枝
    生根率
    Rooting rate of etiolated shoots/%
    未黄化嫩枝
    生根率
    Rooting rate of non-etiolated shoots/%
    黄化嫩枝平均
    生根数
    Average rooting number of etiolated shoots
    未黄化嫩枝平均
    生根数
    Average rooting number of non-etiolated shoots
    黄化嫩枝
    平均根长
    Mean root length of etiolated shoots/cm
    未黄化嫩枝
    平均根长
    Mean root length of non-etiolated shoots/cm
    黄化嫩枝
    平均根径
    Mean root diameter of etiolated shoots/mm
    未黄化嫩枝
    平均根径
    Mean root diameter of non-etiolated shoots/mm
    清水对照
    Water control
    0 0 0 0 0 0 0 0
    3 000 mg/L NAA 0 0 0 0 0 0 0 0
    2 000 mg/L NAA 20.0 16.0 1.00 ± 0.20 1.00 ± 0.1 1.40 ± 0.30 1.00 ± 0.12 0.20 ± 0.08 0.15 ± 0.09
    1 500 mg/L NAA 90.0 75.0 3.70 ± 0.29 1.50 ± 0.25 3.40 ± 0.41 1.20 ± 0.31 0.20 ± 0.04 0.16 ± 0.03
    750 mg/L NAA 25.0 17.0 3.00 ± 0.30 1.10 ± 0.33 2.50 ± 0.60 1.10 ± 0.40 0.20 ± 0.06 0.13 ± 0.04
    600 mg/L NAA 2.0 0 1.50 ± 0.10 0 1.20 ± 0.80 0 0.20 ± 0.07 0
    400 mg/L NAA 5.0 0 2.50 ± 0.50 0 1.20 ± 0.50 0 0.20 ± 0.05 0
    下载: 导出CSV

    表  2  不同质量浓度IBA对栓皮栎黄化和未黄化嫩枝扦插生根的影响

    Table  2.   Effects of different mass concentrations of IBA on rooting of etiolated and non-etiolated shoots of Quercus variabilis

    处理条件
    Treatment
    condition
    黄化嫩枝
    生根率
    Rooting rate of etiolated shoots/%
    未黄化嫩枝
    生根率
    Rooting rate of non-etiolated shoots/%
    黄化嫩枝平均
    生根数
    Average rooting number of etiolated shoots
    未黄化嫩枝平均
    生根数
    Average rooting number of non-etiolated shoots
    黄化嫩枝
    平均根长
    Mean root length of etiolated shoots/cm
    未黄化嫩枝
    平均根长
    Mean root length of non-etiolated shoots/cm
    黄化嫩枝
    平均根径
    Mean root diameter of etiolated shoots/mm
    未黄化嫩枝
    平均根径
    Mean root diameter of non-etiolated shoots/mm
    清水对照
    Water control
    0 0 0 0 0 0 0 0
    3 000 mg/L NAA 0 0 0 0 0 0 0 0
    2 000 mg/L NAA 12.5 0 2.90 ± 0.50 0 2.90 ± 0.20 0 0.12 ± 0.02 0
    1 500 mg/L NAA 100.0 80.0 5.20 ± 0.23 4.10 ± 0.20 5.20 ± 0.40 4.40 ± 0.40 0.16 ± 0.02 0.14 ± 0.02
    750 mg/L NAA 54.0 46.0 4.40 ± 0.34 3.20 ± 0.80 4.40 ± 0.20 3.80 ± 0.30 0.15 ± 0.04 0.14 ± 0.03
    600 mg/L NAA 11.0 8.0 2.10 ± 0.50 2.90 ± 0.40 2.80 ± 0.80 1.60 ± 0.20 0.15 ± 0.10 0.13 ± 0.05
    400 mg/L NAA 10.0 5.0 2.20 ± 0.70 2.20 ± 0.90 3.90 ± 0.60 2.60 ± 0.94 0.13 ± 0.03 0.13 ± 0.01
    下载: 导出CSV

    表  3  不同质量浓度NAA对蒙古栎黄化和未黄化嫩枝扦插生根的影响

    Table  3.   Effects of different mass concentrations of NAA on rooting of etiolated and non-etiolated shoots of Quercus mongolica

    处理条件
    Treatment
    condition
    黄化嫩枝
    生根率
    Rooting rate of etiolated shoots/%
    未黄化嫩枝
    生根率
    Rooting rate of non-etiolated shoots/%
    黄化嫩枝平均
    生根数
    Average rooting number of etiolated shoots
    未黄化嫩枝
    平均生根数
    Average rooting number of non-etiolated shoots
    黄化嫩枝
    平均根长
    Mean root length of etiolated shoots/cm
    未黄化嫩枝
    平均根长
    Mean root length of non-etiolated shoots/cm
    黄化嫩枝
    平均根径
    Mean root diameter of etiolated shoots/mm
    未黄化嫩枝
    平均根径
    Mean root diameter of non-etiolated shoots/mm
    清水对照
    Water control
    0 0 0 0 0 0 0 0
    3 000 mg/L NAA 0 0 0 0 0 0 0 0
    2 000 mg/L NAA 11.0 0 1.30 ± 0.70 0 2.20 ± 0.20 0 0.20 ± 0.02 0
    1 500 mg/L NAA 43.0 30.0 5.50 ± 0.20 2.30 ± 0.10 3.70 ± 0.60 2.60 ± 0.10 0.30 ± 0.04 0.20 ± 0.01
    750 mg/L NAA 27.0 5.0 4.10 ± 0.40 1.90 ± 0.40 3.60 ± 0.20 1.60 ± 0.40 0.20 ± 0.03 0.20 ± 0.02
    600 mg/L NAA 12.0 0 3.10 ± 0.30 0 3.00 ± 0.18 0 0.20 ± 0.06 0
    400 mg/L NAA 5.0 0 1.20 ± 0.50 0 1.10 ± 0.50 0 0.19 ± 0.07 0
    下载: 导出CSV

    表  4  不同质量浓度IBA对蒙古栎黄化和未黄化嫩枝扦插生根的影响

    Table  4.   Effects of different mass concentrations of IBA on rooting of etiolated and non-etiolated shoots of Quercus mongolica

    处理条件
    Treatment
    condition
    黄化嫩枝
    生根率
    Rooting rate of etiolated shoots/%
    未黄化嫩枝
    生根率
    Rooting rate of non-etiolated shoots/%
    黄化嫩枝平均
    生根数
    Average rooting number of etiolated shoots
    未黄化嫩枝
    平均生根数
    Average rooting number of non-etiolated shoots
    黄化嫩枝
    平均根长
    Mean root length of etiolated shoots/cm
    未黄化嫩枝
    平均根长
    Mean root length of non-etiolated shoots/cm
    黄化嫩枝
    平均根径
    Mean root diameter of etiolated shoots/mm
    未黄化嫩枝
    平均根径
    Mean root diameter of non-etiolated shoots/mm
    清水对照
    Water control
    0 0 0 0 0 0 0 0
    3 000 mg/L NAA 0 0 0 0 0 0 0 0
    2 000 mg/L NAA 20.0 16.0 2.80 ± 0.26 1.0 ± 0.20 3.80 ± 0.80 2.50 ± 0.30 0.13 ± 0.05 0.13 ± 0.02
    1 500 mg/L NAA 84.6 70.0 4.80 ± 0.60 1.80 ± 0.10 6.30 ± 0.60 4.60 ± 0.50 0.15 ± 0.01 0.14 ± 0.03
    750 mg/L NAA 10.0 8.0 2.50 ± 0.20 1.50 ± 0.30 3.60 ± 0.50 3.20 ± 0.40 0.14 ± 0.09 0.13 ± 0.06
    600 mg/L NAA 6.0 5.0 2.00 ± 0.10 1.30 ± 0.80 3.50 ± 0.30 1.10 ± 0.20 0.11 ± 0.06 0.13 ± 0.08
    400 mg/L NAA 9.0 4.5 2.00 ± 0.70 1.24 ± 0.50 3.50 ± 0.40 1.00 ± 0.10 0.13 ± 0.03 0.02 ± 0.04
    下载: 导出CSV
  • [1] 冯健. 我国栎类遗传育种研究进展[J]. 辽宁林业科技, 2015(1):43−47. doi: 10.3969/j.issn.1001-1714.2015.01.014

    Feng J. Advances in Quercus genetics and breeding in China[J]. Liaoning Forestry Science and Technology, 2015(1): 43−47. doi: 10.3969/j.issn.1001-1714.2015.01.014
    [2] 卢楠, 孟丙南, 孙宇涵, 等. 四倍体刺槐黄化嫩枝扦插生根过程生理生化分析[J]. 东北林业大学学报, 2013, 41(11):5−9. doi: 10.3969/j.issn.1000-5382.2013.11.002

    Lu N, Meng B N, Sun Y H, et al. Physiology and biochemistry during etiolated shoots cutting of tetraploid Robinia pseudoacacia[J]. Journal of Northeast Forestry University, 2013, 41(11): 5−9. doi: 10.3969/j.issn.1000-5382.2013.11.002
    [3] 裴东. 核桃不定根发生调控机制与蛋白组学探讨[D]. 北京: 北京林业大学, 2004.

    Pei D. Regulation mechanism and proteomics of adventitious roots in walnut[D]. Beijing: Beijing Forestry University, 2004.
    [4] Henrique A, Campinhos E N, Ono E O, et al. Technetium-99m ceftizoxime kit preparation[J]. Brazilian Archives of Biology and Technology, 2006, 49(2): 189−196.
    [5] 丁彤. 北美红栎无性繁殖体系的研究[D]. 合肥: 安徽农业大学, 2012.

    Ding T. Study on asexual reproduction system of northern red oak[D]. Hefei: Anhui Agricultural University, 2012.
    [6] 闫文涛, 佟兆庆, 魏俊, 等. 不同生长调节剂对蒙古栎嫩枝扦插生根的影响[J]. 西北林学院学报, 2017, 32(3):116−121. doi: 10.3969/j.issn.1001-7461.2017.03.22

    Yan W T, Tong Z Q, Wei J, et al. Cuttage propagation technique and rooting mechanism of Quercus mongolica with softwood cutting[J]. Journal of Northwest Forestry University, 2017, 32(3): 116−121. doi: 10.3969/j.issn.1001-7461.2017.03.22
    [7] 刘家财, 滕士元, 史骥清. 弗吉尼亚栎的引种与扦插繁殖生产试验研究[J]. 中国园艺文摘, 2011, 27(6):24−25. doi: 10.3969/j.issn.1672-0873.2011.06.010

    Liu J C, Teng S Y, Shi J Q. Study on introduction and cuttage propagation of Quercus virginiana in Wujiang City, China[J]. Chinese Horticultural Abstracts, 2011, 27(6): 24−25. doi: 10.3969/j.issn.1672-0873.2011.06.010
    [8] 吴幸连. 大叶栎扦插繁殖技术研究[J]. 防护林科技, 2008(5):25−26. doi: 10.3969/j.issn.1005-5215.2008.05.010

    Wu X L. Cutting propagation of Castanopsis fissa[J]. Protective Forest Technology, 2008(5): 25−26. doi: 10.3969/j.issn.1005-5215.2008.05.010
    [9] 吕秀立, 杨芳, 施季森, 等. 苏玛栎无性繁殖试验[J]. 上海交通大学学报(农业科学版), 2013, 31(3):58−62. doi: 10.3969/J.ISSN.1671-9964.2013.03.010

    Lü X L, Yang F, Shi J S, et al. Asexual reproduction experiment of Quercus shumardii[J]. Journal of Shanghai Jiaotong University (Agricultural Science Edition), 2013, 31(3): 58−62. doi: 10.3969/J.ISSN.1671-9964.2013.03.010
    [10] 胡婉仪, 涂炳冲. 栓皮栎、麻栎、小叶栎、苦槠、石栎扦插繁殖简报[J]. 湖北林业科技, 1992(2):35−36.

    Hu W Y, Tu B C. Brief report on cuttage propagation of Quercus variabilis, Quercus acutissima, Quercus chenil, Castanopsis sclerophylla, Lithocrpus glaber[J]. Forestry Science and Technology of Hubei, 1992(2): 35−36.
    [11] 陈丽英, 祁树安. 激素种类、浓度及浸泡时间对红举扦插育苗的影响[J]. 山东农业科学, 2018, 50(8):72−76.

    Chen L Y, Qi S A. Influences of hormone types, concentrations and soaking time on cutting propagation of Zelkova schneideriana[J]. Shandong Agricultural Science, 2018, 50(8): 72−76.
    [12] 故丽米热·卡克什, 木合塔尔·扎热, 马合木提·阿不来提, 等. 不同浓度IBA对大果榛子不同品种黄化萌条扦插成活率影响综合评价[J]. 新疆农业科学, 2018, 55(3):468−476.

    Kakix G, Zari M, Ablat M, et al. Comprehensive evaluation of different concentration IBA effects on the survival rate of yellow sprouted bud cuttings of different big fruit hazelnut cultivars[J]. Xinjiang Agricultural Sciences, 2018, 55(3): 468−476.
    [13] 张乐华, 王书胜, 单文, 等. 基制、激素种类及其浓度对鹿角杜鹃扦插育苗的影响[J]. 林业科学, 2014, 50(3):45−54. doi: 10.3969/j.issn.1672-8246.2014.03.010

    Zhang L H, Wang S S, Shan W, et al. Influences of growth media, and hormone types and concentrations on cutting propagation of Rhododendron latoucheae[J]. Scientia Silvae Sinicae, 2014, 50(3): 45−54. doi: 10.3969/j.issn.1672-8246.2014.03.010
    [14] Shi C, Liu Y, Hu C. Research on hardy branch cutting cultivation of Qinghai spruce[J]. Acta Agriculturae Universitis Jiangxiensis, 2002, 24(2): 259−263.
    [15] Husen A, Pal M. Metabolic changes during adventitious root primordium development in Tectona grandis, Linn. f. (teak) cuttings as affected by age of donor plants and auxin (IBA and NAA) treatment[J]. New Forests, 2007, 33(3): 309−323. doi: 10.1007/s11056-006-9030-7
    [16] 郭素娟. 林木扦插生根的解剖学及生理学研究进展[J]. 北京林业大学学报, 1997, 19(4):64−69. doi: 10.3321/j.issn:1000-1522.1997.04.011

    Guo S J. Progress of study on rooting anatomy and physiology of forest tree cuttings[J]. Journal of Beijing Forestry University, 1997, 19(4): 64−69. doi: 10.3321/j.issn:1000-1522.1997.04.011
    [17] 王艳晶, 彭祚登. 不同生根促进剂对国槐嫩枝扦插生根过程中内源激素变化的影响[J]. 西北林学院学报, 2017, 32(5):109−114. doi: 10.3969/j.issn.1001-7461.2017.05.19

    Wang Y J, Peng Z D. Effects of different rooting accelerators on changes of endogenous hormones in Sophora japonica during softwood-cutting rooting process[J]. Journal of Northwest Forestry University, 2017, 32(5): 109−114. doi: 10.3969/j.issn.1001-7461.2017.05.19
    [18] 申展. 闽楠无性繁殖技术研究[D]. 长沙: 中南林业科技大学, 2013.

    Shen Z. Asexual propagation techniques of Phoebe bournei[D]. Changsha: Central South University of Forestry and Technology, 2013.
    [19] 高茜茜, 刘苑秋, 郭圣茂, 等. 扦插环境及基质对晚松嫩枝扦插生根的影响[J]. 林业科技通讯, 2018(2):31−34.

    Gao Q Q, Liu Y Q, Guo S M, et al. Effects of cutting environment and substrate on rooting of Pinus rigida Mill. var. serotina cuttings[J]. Forestry Science and Technology Newsletter, 2018(2): 31−34.
    [20] 裴东, 谷瑞升. 树木复幼的研究概述[J]. 植物学通报, 2005, 22(6):753−760. doi: 10.3969/j.issn.1674-3466.2005.06.016

    Pei D, Gu R S. A review on the rejuvenation of mature trees[J]. Chinese Bulletin of Botany, 2005, 22(6): 753−760. doi: 10.3969/j.issn.1674-3466.2005.06.016
  • 加载中
图(8) / 表(4)
计量
  • 文章访问数:  1291
  • HTML全文浏览量:  666
  • PDF下载量:  63
  • 被引次数: 0
出版历程
  • 收稿日期:  2019-02-28
  • 修回日期:  2019-06-04
  • 网络出版日期:  2019-07-04
  • 刊出日期:  2019-07-01

目录

    /

    返回文章
    返回