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茎部形成层赤霉素在植物生长发育中的调控作用

杨钧贺 刘畅 钮世辉 李伟

杨钧贺, 刘畅, 钮世辉, 李伟. 茎部形成层赤霉素在植物生长发育中的调控作用[J]. 北京林业大学学报, 2019, 41(7): 68-74. doi: 10.13332/j.1000-1522.20190107
引用本文: 杨钧贺, 刘畅, 钮世辉, 李伟. 茎部形成层赤霉素在植物生长发育中的调控作用[J]. 北京林业大学学报, 2019, 41(7): 68-74. doi: 10.13332/j.1000-1522.20190107
Yang Junhe, Liu Chang, Niu Shihui, Li Wei. Regulatory effect of stem cambium gibberellin on plant growth and development[J]. Journal of Beijing Forestry University, 2019, 41(7): 68-74. doi: 10.13332/j.1000-1522.20190107
Citation: Yang Junhe, Liu Chang, Niu Shihui, Li Wei. Regulatory effect of stem cambium gibberellin on plant growth and development[J]. Journal of Beijing Forestry University, 2019, 41(7): 68-74. doi: 10.13332/j.1000-1522.20190107

茎部形成层赤霉素在植物生长发育中的调控作用

doi: 10.13332/j.1000-1522.20190107
基金项目: 国家自然科学基金项目(31770713,31600535)
详细信息
    作者简介:

    杨钧贺。主要研究方向:林木生物技术。 Email:1092877903@qq.com 地址:100083 北京市海淀区清华东路35号北京林业大学生物科学与技术学院

    责任作者:

    李伟,教授,博士生导师。主要研究方向:针叶树遗传改良。Email:bjfuliwei@bjfu.edu.cn 地址:同上

  • 中图分类号: Q946.885+.5; S 792.95

Regulatory effect of stem cambium gibberellin on plant growth and development

  • 摘要: 目的赤霉素(GAs)是一种重要的植物激素,对植物生长发育具有广泛的调控作用。本文通过对茎部形成层赤霉素在植物生长发育中的调控作用进行研究,为阐明植物体内源活性GAs的运输及其调控作用提供参考。方法本研究通过转基因方法特异下调烟草茎部形成层活性GAs含量,探究其对植株茎部、叶片、根生长发育的调控作用;在此基础上,利用35S:: PtGA20ox、35S:: PtGA2ox1转基因烟草株系分别作接穗和砧木进行微嫁接和施加外源GAs,探究内源与外源活性GAs在烟草体内的运输特性。结果结果表明,茎部形成层特异启动子LMX5介导的PtGA2ox1转基因烟草植株茎部GAs含量与野生型相比显著降低,而叶与根中含量无明显变化;通过石蜡切片观察发现茎部形成层GAs含量降低明显延缓了茎部木质部的发育,同时对植物的叶片、侧根的生长也有抑制作用;对不同转基因株系微嫁接分析发现,外源施加的GAs可以恢复茎部形成层GAs缺乏引起的表型,而内源活性GAs却不能自由运输,也不能恢复表型;结论茎部形成层GAs含量不仅对茎部木质部的发育有重要作用,并且对叶片的生长及不定根的发育也有调控作用,利用微嫁接的方法验证了活性GAs在烟草中的运输机制,明确了GAs在调控植物组织发育中的重要意义,为今后GAs在林木中的应用奠定了理论基础。

     

  • 图  1  不同烟草植株及基因表达水平

    A是不同烟草植株,标尺长度为1 cm;B是转基因株系各组织中PtGA2ox1基因的表达水平;C是转基因株系不同组织中烟草NtGA2ox1基因本底表达水平,将35:G2植株中的PtGA2ox1基因的表达量设置为1。A is different tobacco plant with a scale length of 1 cm; B is the expression level of PtGA2ox1 gene in each tissue of transgenic lines; C is the background expression level of tobacco NtGA2ox1 gene in different tissues of transgenic lines, which will be in 35:G2 plants, the expression level of the PtGA2ox1 gene was set to 1.

    Figure  1.  Different tobacco plants and gene expression levels

    图  2  转基因植株根部、茎部、叶片中GAs的含量

    A. 转基因植株根部GAs的含量;B. 转基因植株茎部GAs的含量;C. 转基因植株叶片中GAs的含量。A, content of GAs in roots of transgenic plants; B, content of GAs in the stem of transgenic plants; C, content of GAs in leaves of transgenic plants.

    Figure  2.  GAs content in stems, roots and leaves of transgenic plants

    图  3  不同基因型组成的嫁接苗和不同基因型的烟草植株

    A.普通MS培养基中的WT//WT嫁接植株; B.添加有GA4+7的WT//WT嫁接植株; C.普通MS培养基中的35:G2//35:G20嫁接植株; D.普通MS培养基中的35:G20//35:G2嫁接植株; E.不同基因型的烟草植株。箭头代表嫁接处;A ~ D标尺长度为2 cm;E标尺长度为1 cm。The E scale is 1 cm in length; A,WT//WT grafted plants in normal MS medium; B,WT//WT grafted plants with GA4+7 were added; C, G2//35: G20 grafted plants in normal MS medium; D, 35:G20//35:G2 grafted plants in normal MS medium; E,different genotypes of tobacco plants. The arrow represents the graft; the A−D scale is 2 cm in length.

    Figure  3.  Grafted seedlings of different genotypes and tobacco plants of different genotypes

    图  4  内源调控GAs含量对植株木质部发育的影响

    PV. 初生导管组织;C. 形成层;X. 木质部;P. 韧皮部。标尺长度为100 μm。PV, primary ductal tissue; C, forming layer; X, xylem; P, phloem. Scale length is 100 μm.

    Figure  4.  Effects of modification of endogenous GAs levels on xylem development

    图  5  内源调控GAs含量对植株叶片发育的影响

    每行分别为各植株的全株叶片;标尺长度为1 cm。Each row is the whole plant leaf of each plant; the length of the scale is 1 cm.

    Figure  5.  Effects of endogenous regulation of GAs content on leaf development of plants

    图  6  内源调控GAs含量对植株根生长发育的影响

    A、B为野生型和转基因植物不定根的平均数量和平均长度;C ~ F为不同转基因型植株根部,标尺长度为1 cm。C为WT株系;D为35:G2株系;E为35:G20株系;F为L:G2株系。A, B, the number and length of average adventitious roots of wild-type and transgenic plants. C−F, roots of different transgenic plants, the length of the scale is 1 cm. C, WT strain; D, 35: G2 strain; E, 35: G20 strain; F, L: G2 strain.

    Figure  6.  Effects of endogenous regulation of GAs content on root growth and development of plants

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出版历程
  • 收稿日期:  2019-02-28
  • 修回日期:  2019-05-30
  • 网络出版日期:  2019-07-05
  • 刊出日期:  2019-07-01

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