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文冠果FAD2的序列与功能分析

赵娜 张媛 李秋琦 李茹芳 郭惠红

赵娜, 张媛, 李秋琦, 李茹芳, 郭惠红. 文冠果FAD2的序列与功能分析[J]. 北京林业大学学报, 2015, 37(2): 87-93. doi: 10.13332/j.cnki.jbfu.2015.02.017
引用本文: 赵娜, 张媛, 李秋琦, 李茹芳, 郭惠红. 文冠果FAD2的序列与功能分析[J]. 北京林业大学学报, 2015, 37(2): 87-93. doi: 10.13332/j.cnki.jbfu.2015.02.017
ZHAO Na, ZHANG Yuan, LI Qiu-qi, LI Ru-fang, GUO Hui-hong. Sequence and functional analysis of FAD2 gene from Xanthoceras sorbifolia seeds[J]. Journal of Beijing Forestry University, 2015, 37(2): 87-93. doi: 10.13332/j.cnki.jbfu.2015.02.017
Citation: ZHAO Na, ZHANG Yuan, LI Qiu-qi, LI Ru-fang, GUO Hui-hong. Sequence and functional analysis of FAD2 gene from Xanthoceras sorbifolia seeds[J]. Journal of Beijing Forestry University, 2015, 37(2): 87-93. doi: 10.13332/j.cnki.jbfu.2015.02.017

文冠果FAD2的序列与功能分析

doi: 10.13332/j.cnki.jbfu.2015.02.017
基金项目: 

国家自然科学基金项目(J1103516)。

详细信息
    责任作者:

    第一作者: 赵娜。主要研究方向:植物发育生物学。Email: momozhaona@163.com 地址: 100083 北京市清华东路35号北京林业大学生物科学与技术学院162信箱。 责任作者: 郭惠红,副教授。主要研究方向:植物发育生物学。 Email: guohh@bjfu.edu.cn 地址:同上。

    第一作者: 赵娜。主要研究方向:植物发育生物学。Email: momozhaona@163.com 地址: 100083 北京市清华东路35号北京林业大学生物科学与技术学院162信箱。 责任作者: 郭惠红,副教授。主要研究方向:植物发育生物学。 Email: guohh@bjfu.edu.cn 地址:同上。

Sequence and functional analysis of FAD2 gene from Xanthoceras sorbifolia seeds

  • 摘要: 脂肪酸脱饱和酶2(fatty acid desaturase 2, FAD2)基因是油脂合成代谢的关键酶基因,其编码的蛋白催化油酸(18∶1)进一步脱饱和形成亚油酸(18∶2)。本研究以富含油酸和亚油酸2种优质不饱和脂肪酸的文冠果种胚为试材,采用简并引物策略结合RACE技术,克隆了文冠果FAD2的cDNA序列。序列分析表明,文冠果FAD2除具有植物FAD2特有的3个组氨酸区和N端的芳香族氨基酸区外,还具有3个N—糖基化位点和多个磷酸化位点。通过农杆菌介导,将文冠果FAD2转入拟南芥fad2突变体中进行表达分析。气相结果表明,拟南芥fad2突变体缺失亚油酸,而转入文冠果FAD2的拟南芥fad2突变体的种子油中产生了亚油酸,这表明所克隆的文冠果FAD2基因编码的酶具有催化油酸(18∶1)脱饱和成为亚油酸(18∶2)的活性。

     

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出版历程
  • 收稿日期:  2014-06-04

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