Effects of gibberellic acid and chilling treatments on seed germination and seedling growth of Paeonia rockii hybrids
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摘要: 本文研究了在容器栽培条件下,赤霉素(GA3)处理对紫斑牡丹种子生根的影响,不同主根长度、低温((3±1)℃)时间、赤霉素(GA3)浓度对种子发芽和幼苗生长的影响。结果表明:GA3能够促进种子生根,其中质量浓度为100mg/L的处理效果最好;种子发芽过程中,主根长度与低温时间的交互效应对初萌期和发芽率存在极显著的影响;只有在主根生长超过40mm时,低温和GA3处理才能有效地促进种子发芽,在此基础上,低温14d结合100、200mg/L 或者500mg/L GA3处理为促进发芽的较好组合。当主根长度超过40mm时,低温和GA3处理才能有效地促进幼苗的生长,以低温14d结合500mg/L GA3处理为最佳组合。综合分析表明,紫斑牡丹优化的种子播种育苗程序为:用100mg/L GA3处理种子24h后室温沙藏,待主根生长超过40mm时播种,并置于低温(3±1)℃下处理14d,再用500mg/L 的GA3处理后,于温室中培养成苗。Abstract: We explored the effects of gibberellic acid (GA3) in releasing seed hypocotyl dormancy of Paeonia rockii hybrids, and investigated the effects of root length, duration of the chilling treatment ((3±1) ℃) and GA3 concentration on the epicotyl dormancy breaking and seedling growth. Results showed that 100 mg/L GA3 treatment was the best to promote rooting. During the process of seed germination, interaction of root length and chilling treatment had a significant effect on the first sprouting time and germination rate. GA3 and chilling treatment could effectively promote the germination of seeds with radicle roots longer than 40 mm; on this basis, the chilling treatment for 14 d, combined respectively with 100, 200 mg/L GA3 or 500 mg/L GA3, was in favor of seed germination. GA3 and chilling treatment could also promote effectively the growth of seedlings with radicle roots longer than 40 mm, and the chilling treatment for 14 d, combined with 500 mg/L GA3, was optimum for seedling growth. We summarized the optimum procedure for seed germination as follows. First, the seeds should be soaked in 100 mg/L GA3 solution for 24 h and stratified in sand until roots grew up to 40 mm in length. Then they should be sowed in plug trays under the chilling treatment for 14 d. Finally, after being watered with 500 mg/L GA3 solution, they can grow up into seedlings under the greenhouse conditions.
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