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白桦雌花发育前期和中期差异基因表达分析

赵丽红 周菲 官民晓 刘闯 赵鑫 刘雪梅

赵丽红, 周菲, 官民晓, 刘闯, 赵鑫, 刘雪梅. 白桦雌花发育前期和中期差异基因表达分析[J]. 北京林业大学学报, 2015, 37(4): 104-112. doi: DOI:10.13332/j.1000-1522.20140301
引用本文: 赵丽红, 周菲, 官民晓, 刘闯, 赵鑫, 刘雪梅. 白桦雌花发育前期和中期差异基因表达分析[J]. 北京林业大学学报, 2015, 37(4): 104-112. doi: DOI:10.13332/j.1000-1522.20140301
ZHAO Li-hong, ZHOU Fei, GUAN Min-xiao, LIU Chuang, ZHAO Xin, LIU Xue-mei. Analysis of differentially expressed transcripts in early and middle development of Betula platyphylla female inflorescence[J]. Journal of Beijing Forestry University, 2015, 37(4): 104-112. doi: DOI:10.13332/j.1000-1522.20140301
Citation: ZHAO Li-hong, ZHOU Fei, GUAN Min-xiao, LIU Chuang, ZHAO Xin, LIU Xue-mei. Analysis of differentially expressed transcripts in early and middle development of Betula platyphylla female inflorescence[J]. Journal of Beijing Forestry University, 2015, 37(4): 104-112. doi: DOI:10.13332/j.1000-1522.20140301

白桦雌花发育前期和中期差异基因表达分析

doi: DOI:10.13332/j.1000-1522.20140301
基金项目: 

中央高校基本科研费专项(DL12EA02)、国家自然科学基金项目(31100449)、国家基础科学人才培养基金项目(J1210053)。

详细信息
    作者简介:

    第一作者: 赵丽红。主要研究方向:植物遗传与发育。Email: 540211317@qq.com 地址:150040 黑龙江省哈尔滨市和兴路26号东北林业大学生命科学学院。责任作者: 刘雪梅,博士,教授。主要研究方向:植物遗传与发育。Email: 695898040@qq.com 地址:同上。

    第一作者: 赵丽红。主要研究方向:植物遗传与发育。Email: 540211317@qq.com 地址:150040 黑龙江省哈尔滨市和兴路26号东北林业大学生命科学学院。责任作者: 刘雪梅,博士,教授。主要研究方向:植物遗传与发育。Email: 695898040@qq.com 地址:同上。

Analysis of differentially expressed transcripts in early and middle development of Betula platyphylla female inflorescence

  • 摘要: 白桦为雌雄同株的单性花树种,在木本植物花发育研究中有着重要作用。本文旨在初步确定白桦雌花发育过程中的关键时期大孢子发生(前期)和配子体发育(中期)过程中基因表达及调控的差异,同时挖掘与雌花发育相关的基因。首先,运用细胞学方法确定试材雌花的发育时期。其次,采用抑制消减杂交(SSH)技术,分别以白桦前期和中期雌花序的cDNA作为tester和driver,建立正、反向抑制消减杂交cDNA文库:BHHQ和BHHZ。共获得1406个高质量的EST序列,其中787个与数据库中的已知核酸或蛋白序列有较高同源性, 47个可能与花发育相关。GO分析结果表明,这些基因34.96%参与生物合成,16.45%参与细胞组件,48.59%参与分子功能。另外,选择6个与花发育相关EST进行qRT-PCR分析,结果显示这些基因在不同组织中都有一定表达。其中bhhq.408,bhhq.435,bhhz.359,bhhz.215在生殖器官中的表达明显高于营养器官;bhhq.254,bhhq.190在绝大多数组织中均有一定表达,说明这些基因不仅参与生殖器官的发育,也在营养器官中发挥一定的作用。

     

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出版历程
  • 收稿日期:  2014-09-04
  • 修回日期:  2014-12-09
  • 刊出日期:  2015-04-30

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