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盐胁迫对转SacB、BADH基因的美丽胡枝子部分逆境生理指标的影响

杨晓红,  陈晓阳,  李守菊,  隗晓丹,  王颖,  周伟, 

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文章导航 > 北京林业大学学报  > 2009 >  31(4): 36-40
杨晓红, 陈晓阳, 李守菊, 隗晓丹, 王颖, 周伟, . 盐胁迫对转SacB、BADH基因的美丽胡枝子部分逆境生理指标的影响[J]. 北京林业大学学报, 2009, 31(4): 36-40.
引用本文: 杨晓红, 陈晓阳, 李守菊, 隗晓丹, 王颖, 周伟, . 盐胁迫对转SacB、BADH基因的美丽胡枝子部分逆境生理指标的影响[J]. 北京林业大学学报, 2009, 31(4): 36-40.
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YANG Xiao-hong, , CHEN Xiao-yang, LI Shou-ju, WEI Xiao-dan, WANG Ying, ZHOU Wei. Effec ts of salt stress on some stress physiological indexes of SacBtransgenic BADHtransgenic Lespedeza formosa plants.[J]. Journal of Beijing Forestry University, 2009, 31(4): 36-40.
Citation: YANG Xiao-hong, , CHEN Xiao-yang, LI Shou-ju, WEI Xiao-dan, WANG Ying, ZHOU Wei. Effec ts of salt stress on some stress physiological indexes of SacBtransgenic BADHtransgenic Lespedeza formosa plants.[J]. Journal of Beijing Forestry University, 2009, 31(4): 36-40.
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盐胁迫对转SacB、BADH基因的美丽胡枝子部分逆境生理指标的影响

  • 1.

    1 北京林业大学林木花卉遗传育种教育部重点实验室 2 北京农学院园林系 3华南农业大学林学院

Effec ts of salt stress on some stress physiological indexes of SacBtransgenic BADHtransgenic Lespedeza formosa plants.

  • 1.

    1 Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University,100083, P.R. China; 2 Landscape Department, Beijing University of Agriculture, 102206, P.R. China; 3 College of Forestry, South China Agricultural University, Guangzhou,510642, P.R. China.

  • 摘要: 为了探讨盐胁迫下外源基因对植物渗透调节的影响，以同时培育的转入果聚糖蔗糖转移酶（SacB）基因、转甜菜碱醛脱氢酶（BADH）基因及未转基因的美丽胡枝子盆栽苗为材料，研究不同浓度(0、0.5%、1.0%、2.0%)NaCl处理对3种试验材料的耐盐性及盐胁迫下的脯氨酸、甜菜碱、可溶性糖、丙二醛含量、过氧化物歧化酶活性的影响。结果表明：在未进行盐胁迫时，3种试材的这几项指标含量没有明显差异，但随着盐胁迫强度的增加，两种转基因的美丽胡枝子在积累脯氨酸、可溶性糖能力方面明显强于非转基因植株，转入BADH基因的美丽胡枝子在积累甜菜碱上要强于非转基因植株及转入SacB基因的植株；尽管在盐胁迫强度增大的情况下，3种植株的过氧化物歧化酶活性增强了，但两种转基因植株的过氧化物歧化酶活性并没有明显大于非转基因植株；两种转基因植株可明显抑制丙二醛在植物体内的快速积累。
    Abstract: SacBtransgenic, BADHtransgenic and nontransgenicLespedeza formosa potted plants were selected to explore the effects of exogenous gene on osmotic regulation of plants under salt stress. All plants were cultured under same conditions and were treated with NaCl solutions. Salt stress tolerance of 3 types of plant materials was compared. Several physiological indexes, including proline, betaine, malonalohyde (MDA), superoxide dismutase (SOD) and soluble sugar were assayed when three L. formosa material types were treated with 4 NaCl concentrations. The concentration gradients of NaCl solution were 0, 0.5%, 1.0% and 2.0%. The results showed that there were no significant differences among aforementioned indexes of 3 material types when plants were free from salt stress. With the increase of NaCl concentration, the transgenic plants accumulated higher levels of proline and soluble sugar than wildtype plants. The BADHtransgenic L. formosa accumulated higher levels of betaine than SacBtransgenic and nontransgenic plants. The SacBtransgenic L. formosa accumulated almost the same levels of betaine as the wildtype plants during salt stress. With the increase of salt concentration, all 3 types showed higher SOD activity, but there was no significant difference in SOD activity among them. The levels of MDA in plants with expressing SacB gene or BADHgene were lower than nontransgenic plants under saltstress treatment. 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出版历程
  • 收稿日期:  1900-01-01
  • 修回日期:  1900-01-01
  • 刊出日期:  2009-07-29

盐胁迫对转SacB、BADH基因的美丽胡枝子部分逆境生理指标的影响

  • 1. 1 北京林业大学林木花卉遗传育种教育部重点实验室 2 北京农学院园林系 3华南农业大学林学院
  • 收稿日期: 1900-01-01
  • 修回日期: 1900-01-01
  • 刊出日期: 2009-07-29

摘要: 为了探讨盐胁迫下外源基因对植物渗透调节的影响，以同时培育的转入果聚糖蔗糖转移酶（SacB）基因、转甜菜碱醛脱氢酶（BADH）基因及未转基因的美丽胡枝子盆栽苗为材料，研究不同浓度(0、0.5%、1.0%、2.0%)NaCl处理对3种试验材料的耐盐性及盐胁迫下的脯氨酸、甜菜碱、可溶性糖、丙二醛含量、过氧化物歧化酶活性的影响。结果表明：在未进行盐胁迫时，3种试材的这几项指标含量没有明显差异，但随着盐胁迫强度的增加，两种转基因的美丽胡枝子在积累脯氨酸、可溶性糖能力方面明显强于非转基因植株，转入BADH基因的美丽胡枝子在积累甜菜碱上要强于非转基因植株及转入SacB基因的植株；尽管在盐胁迫强度增大的情况下，3种植株的过氧化物歧化酶活性增强了，但两种转基因植株的过氧化物歧化酶活性并没有明显大于非转基因植株；两种转基因植株可明显抑制丙二醛在植物体内的快速积累。

English Abstract

杨晓红, 陈晓阳, 李守菊, 隗晓丹, 王颖, 周伟, . 盐胁迫对转SacB、BADH基因的美丽胡枝子部分逆境生理指标的影响[J]. 北京林业大学学报, 2009, 31(4): 36-40.
引用本文: 杨晓红, 陈晓阳, 李守菊, 隗晓丹, 王颖, 周伟, . 盐胁迫对转SacB、BADH基因的美丽胡枝子部分逆境生理指标的影响[J]. 北京林业大学学报, 2009, 31(4): 36-40.
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YANG Xiao-hong, , CHEN Xiao-yang, LI Shou-ju, WEI Xiao-dan, WANG Ying, ZHOU Wei. Effec ts of salt stress on some stress physiological indexes of SacBtransgenic BADHtransgenic Lespedeza formosa plants.[J]. Journal of Beijing Forestry University, 2009, 31(4): 36-40.
Citation: YANG Xiao-hong, , CHEN Xiao-yang, LI Shou-ju, WEI Xiao-dan, WANG Ying, ZHOU Wei. Effec ts of salt stress on some stress physiological indexes of SacBtransgenic BADHtransgenic Lespedeza formosa plants.[J]. Journal of Beijing Forestry University, 2009, 31(4): 36-40.
shu

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