Keywords: 
• Rhododendron hybridum /
• chlorophyll /
• active Fe /
• catalase /
• peroxidase

Abstract: Nitrogen source(NO-3N/NH+4N) and pH are both important factors for plant growth. To determine whether or not the two factors affecting the physiological characteristics, active iron (Fe) and active manganese nutrition of Rhododendron hybridum, threeyearold cultivated R. hybridum plants were treated by nutrition solution with two different pH values (80 and 85) and nitrogen sources (NO－3N/NH+4N). These treatments were cultivated with a completely randomized block design for a sixmonth period in the Zhuoyue Flower Greenhouse in Beijing. Data analysis with SPSS (statistical package for the social sciences) on the physicochemical properties of medium material and active Fe, active Mn, Chlorophyll, catalase activity, peroxidase activity, showed that, when the pH of nutrition solution was 8.0, there was a more significant increase in the pH of NO-3 N treated medium than that of NH+4N treated one. Irrigating nutrient solution with a pH of 50 maintained optimum medium pH range (pH55-45) for R. hybridum. There was no significant differences among different pH and nitrogen source treatments on the effective nutrient composition of the mediummaterial and the appearance of R. hybridum. The new leaves of NH+4N (pH 5.0) treated plants had the highest content of chlorophyll and active Fe, 1.590 mg/g and 26.379 mg/kg on average, respectively. In comparison to the mature leaves, young leaves had lower average content of active Fe, active Mn, and chlorophyll, with almost equal catalase activity and higher peroxidase activity. These results indicate that NH+4 as the nitrogen source improves the effectiveness of iron, and increases the content of chlorophyll in the leaves of R. hybridum, catalase activity and the prevention of chrosis. Hence, NH+4N is a better nitrogen source for R. hybridum than NO－3N in the northern alkaline soil region of China. 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