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燕山板栗叶片基因组AFLP反应体系建立

程丽莉, 苏淑钗, 秦岭, 刘建立, 尹伟伦

程丽莉, 苏淑钗, 秦岭, 刘建立, 尹伟伦. 燕山板栗叶片基因组AFLP反应体系建立[J]. 北京林业大学学报, 2006, 28(6): 35-39.
引用本文: 程丽莉, 苏淑钗, 秦岭, 刘建立, 尹伟伦. 燕山板栗叶片基因组AFLP反应体系建立[J]. 北京林业大学学报, 2006, 28(6): 35-39.
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CHENG Li-li, SU Shu-chai, QIN Ling, LIU Jian-li, YIN Wei-lun. Construction of AFLP reaction system in leaves of Castanea mollissima in the Yanshan Mountains[J]. Journal of Beijing Forestry University, 2006, 28(6): 35-39.
Citation: CHENG Li-li, SU Shu-chai, QIN Ling, LIU Jian-li, YIN Wei-lun. Construction of AFLP reaction system in leaves of Castanea mollissima in the Yanshan Mountains[J]. Journal of Beijing Forestry University, 2006, 28(6): 35-39.
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燕山板栗叶片基因组AFLP反应体系建立

  • 1. 北京林业大学资源与环境学院‚省部共建森林培育与保护教育部重点实验室;

  • 2. 北京市农林科学院林业果树研究所;

  • 3. 北京农学院植物科学技术系;

  • 4. 中国林业科学研究院森林生态环境研究所

基金项目: 

“十五”国家科技攻关项目(2004BA515B06).

详细信息
    作者简介:

    程丽莉,硕士.主要研究方向:果树种质资源学.电话:010-82590742E-mail:lily980101@126.com现工作单位地址:100093北京海淀香山瑞王坟甲12号北京市农林科学院林业果树研究所.

    责任作者:

    苏淑钗,副教授.主要研究方向:果树种质资源学.电话:010-62338128 E-mail:sushuchai@sohu.com 地址:100083 北京林业大学资源与环境学院.

  • 中图分类号: S759.3+4;Q943.2

Construction of AFLP reaction system in leaves of Castanea mollissima in the Yanshan Mountains

  • 1. Key Laboratory for Silviculture and Conservation, Ministry of Education, College of Natural Resources & Environment, Beijing Forestry University, 100083, P. R. China;

  • 2. Institute of Forestry Pomology, Beijing Academy of Agricultural and Forestry Sciences, 100091, P. R. China;

  • 3. Department of Plant Science, Beijing Agricultural College, 102206, P. R. China;

  • 4. Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, 100091, P. R. China

摘要

    摘要
  • 摘要: 该文以燕山板栗嫩叶为材料,利用改良的CTAB法提取高质量的总DNA,通过优化了酶切连接、预扩增、选择性扩增等试验条件,得到了清晰的板栗AFLP指纹图谱.研究结果表明①应用经过改良的CTAB法可获得用于构建板栗AFLP体系的高质量DNA;②单独酶切和单独酶连体系比酶切酶连共体系效果更好,确定了板栗基因组DNAAFLP分子标记反应体系;③在所分析的9种引物组合中EAAC+M-CAA、E-AAC+M-CAT和E-AGT+M-CAT3个引物均获得较好的多态性.其中以E-AGT+M-CAT引物对组合的扩增条带信号强度一致性好,条带分布均匀,得到了稳定、清晰、分辨率较高的指纹谱带.
    Abstract: High quality DNA required for construction of AFLP analysis system was isolated with an improved method of CTAB from young leaves of Castanea mollissima. A silver-staining AFLP reaction system was constructed for genetic analysis of C. mollissima by optimizing DNA restriction-ligase reaction, pre-amplification and selective amplification.Resultsshowed that the system of restriction of DNA was better than restriction and link of DNA. The polymorphism bands were obtained from three primer pairs (E-AAC+M-CAA, E-AAC+M-CAT, E-AGT+M-CAT), but E-AGT+M-CAT could amplify more uniform and high sharp bands. Our study showed that the silver-staining AFLP was available for genetic diversification, variety identification, construction of molecular map, identification and classification.
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出版历程
  • 收稿日期:  2006-09-24
  • 网络出版日期:  2024-05-14

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