Abstract: Wildlife path is one of the most effective ways to slow down the fragmentation of wildlife habitat, which was caused by the rapid development of railway and road. The concept, application objects, design principles, design basis of wildlife path were established, the design requirements of wildlife path parameters (such as location, quantity, form, width, height, ground matrix and environment of the openings) were given. Taken Sixiao Highway Wildlife Path of Yunnan as example, the ratio of Asian elephant path utilization between September 2005 to May 2008 was only 40%, and “the matching degree of path location and the migration routes冶was the decisive factor for Asian elephants. In order to evaluate the effectiveness and ensure the long-term viability of the wildlife path, an uninterrupted monitoring and the reconstruction design with pertinence are advised.