Abstract: Using tissue culture,the effects of cytokinin on the induction of bud and micro-tuber from the color callalily in vitro was studied. The optimal medium for induction of bud and micro-tuber from the color callalily in vitro was selected.Results show that the addition of 6-BA 0.5-1.5 mg/L to the MS medium is most favorable for the induction of shoots and its shoot regeneration frequency is up to 80%; the application of 6-B 2.5-4.0 mg/L in the micro-tuber inducing medium show a marked difference with CK,and the micro-tuber rate also reaches 80%. Therefore 6-BA is regarded as an essential element in the composition of the micro-tuber inducing culture medium, and the best medium of micro-tuber inducing is the MS medium supplemented with 6-BA 2.5 mg/L.