摘要:
该文对福建省光皮桦11个天然群体的RAPD和ISSR的遗传多样性和基因多样性进行分析,选择34个引物(25个RAPD引物,9个ISSR引物),对11个光皮桦群体77个个体进行扩增.RAPD引物共检测到270个位点,多态位点267个,占98.52%.ISSR引物共检测到113个位点,均为多态位点.研究结果表明,11个天然群体中沙县罗卜岩自然保护区和武平梁野山自然保护区群体的遗传多样性和基因多样性最丰富,三元群体的遗传多样性和基因多样性最低;群体内的遗传多样性和基因多样性明显高于群体间的遗传多样性和基因多样性;邵武卫闽和沙县罗卜岩自然保护区群体遗传相似度最低.两种标记的UPGMA聚类分析结果虽存在差异,但都将11个天然群体明显分为两大类.该研究结果为光皮桦树种今后的遗传改良奠定了基础.
Abstract:
Analysis on the genetic diversity of 11 natural populations of Betula luminifera was carried out in Fujian Province.Thirty-four reliable primers(25 RAPD primers, nine ISSR primers) were chosen to amplify DNA samples from 77 individuals of 11 natural populations of B. luminifera. Based on the 25 RAPD primers, a total of 270 bands were generated, among which polymorphic bands accounted for 98.52% and the number is 267. Based on the nine ISSR primers, 113 bands were produced and all of them were polymorphic bands. The results of research showed that among the 11 natural populations, those in Luobuyan Nature Reserve of Shaxian County and Liangye Mountain Nature Reserve of Wuping County had the highest genetic and gene diversity and richness, while the population of Sanyuan County had the least one. Also the genetic and gene diversity within populations was obviously higher than that among the populations. It also showed that the similarity index between Weimin County and Luobuyan Nature Reserve of Shaxian County was the least.Based on RAPD and ISSR markers, the 11 natural populations were divided into two clusters according to the UPGMA cluster analysis, although the differences between RAPD and ISSR markers were observed. All of the results lay a foundation for the future genetic improvement of %B. luminifera%.
百度学术
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