Induction of xylanase from Strepotmyces albus and analysis of thermostability and alkali-tolerance
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摘要: 该文采用白色链霉菌为实验菌种,在不同诱导产酶培养基上经过240h的振荡培养,探索其产酶时程规律.结果表明,不同的诱导底物诱导产生的最大木聚糖酶活不等,其中木粉的诱导效果好于纯木聚糖,复合底物的诱导效果好于单一底物.该菌在筛选出的最佳诱导产酶培养基上培养144h后达到产酶高峰,粗酶液酶活可达到45.66U/mL.对该酶进行高温及碱性处理的结果表明,它具有较好的耐热耐碱性,在pH 7.0下反应表现最高活性,同时在90℃下保温30min后酶活残留达83.46%Abstract: Time course of xylanase' production was studied by culturing Strepotmyces albus in different substrates for 240 h. The results show that xylanase demonstrates different activities in different substrates. For induction of xylanase, wood powder is better than xylan, while a compound substrate is better than a single one. After Strepotmyces albus is cultured for 144 h in the optimal enzyme-induction medium, the production of xylanase culminates, with a maximum enzyme activity of 45.66 U/mL. The results show that the xylanase from Strepotmyces albus has the characteristics of both thermotolerance and alkali-tolerance, showing that 83.46% of its maximal activity remains after a treatment for 30 min at 90℃, and the highest activity in pH 7.0.
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Keywords:
- Strepotmyces albus /
- xylanase /
- thermostability /
- alkali-tolerance
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