Tissue culture of Acer truncatum
-
摘要: 该文对元宝枫茎段组织培养中的外植体灭菌、褐变预防、增殖培养、根诱导等进行了试验研究.结果表明:元宝枫外植体最佳取材时期为4月份,由4年生和20年生母树上取材的外植体,经0.1% HgCl2灭菌6min后,污染率分别降为16.67%和14.29%;对于4龄和20龄母树上取材的外植体进行培养时,在培养基中分别添加500及750mg LPVP,可以较好地控制褐变;当MS培养基中附加0.01~0.05μmol LTDZ时,可有效地促进外植体增殖和生长;适宜的生根培养基为1/2MS+01μmol/LIBA,每天光照16h,生根率可达90.91%;幼苗经炼苗后盆栽,成活率在95%以上.Abstract: The stem segments of Acer truncatum were used as explants to study sterilization techniques,browning prevention,proliferation and rooting in vitro.The results show that the optimum season for explant collection is April,the contamination rates of the explants from the 4- and 20-years-old trees are 16.7% and 14.3% after the explants are sterilized in 0.1% aqueous mercuric chloride for 6 min;for the explants from the 4- and 20-years-old trees the addition of PVP of 500 mg/L and 750 mg/L in the medium can prevent browning effectively.The MS medium containing 0.01~0.05 μmol/L TDZ is most efficient in the proliferation of Acer truncatum.The ratio of rhizogenesis is 90.91% in the optimum rooting medium of 1/2 MS medium supplemented with 0.1 μmol/L IBA and under 16 h of photoperiod;the survival ratio of transplanting is 95% after training seedling.
-
Keywords:
- Acer truncatum /
- tissue culture /
- sterilization /
- browning /
- propagation
-
-
期刊类型引用(4)
1. 杨铭伟，王时英. 超声振动时效机理分析及试验研究. 中国农机化学报. 2016(02): 70-74 . 
百度学术
2. 田超，李学敏，龚习. 航天结构件焊接残余应力的无损检测方法. 科技风. 2015(21): 53-54 . 百度学术
3. 冯爱新，王俊伟，周鹏程，李彬. WC硬质合金管螺纹梳刀应力常数的实验研究. 热加工工艺. 2012(20): 69-71 . 百度学术
4. 张亦良，刘金艳，徐学东. 不锈钢X射线应力测量. 北京工业大学学报. 2010(09): 1165-1173 . 百度学术
其他类型引用(17)
计量
- 文章访问数: 3256
- HTML全文浏览量: 117
- PDF下载量: 87
- 被引次数: 21
下载:
