Abstract: The effects of different concentrations of 2,4-D, NAA and KT on the callus formation and somatic embryogenesis of Cymbidium hybridum were investigated using an orthogonal design. The results show that:1) the effects of 2,4-D, NAA and KT on the frequency of callus formation and somatic embryogenesis of Cymbidium hybridum differ extremely significantly; 2) 2,4-D is a necessary factor to induce callus, and the frequency of callus formation increases with the increase of concentrations, but the explants will turn brown and die when the concentration is above 2.0mg/L; 3) KT is the key factor to induce formation of somatic embryogenesis, and its effects are restrained by 2,4-D and accelerated by a low concentration of NAA; 4) the frequency of somatic embryogenesis formation is also related to the genotype, but its dependence on the genotype has clustering and scattering characteristics.