摘要:
采用正交试验设计方法研究了3种激素2,4-D、萘乙酸(NAA)和激动素(KT)不同浓度对4个不同品种大花蕙兰茎段愈伤组织诱导和体胚发生频率的影响.结果表明:①3种激素对愈伤组织的诱导和体胚发生的影响均达极显著差异;②2,4D对愈伤组织的发生有非常显著的作用,且随浓度升高,诱导率增加,但高于2.0mgL时,外植体褐化死亡;③KT是体胚发生的关键因子,其作用受到2,4-D的抑制和低浓度NAA的促进;④体胚的发生受基因型的影响,但其对基因型的依赖性具分组特性.
Abstract:
The effects of different concentrations of 2,4-D, NAA and KT on the callus formation and somatic embryogenesis of Cymbidium hybridum were investigated using an orthogonal design. The results show that:1) the effects of 2,4-D, NAA and KT on the frequency of callus formation and somatic embryogenesis of Cymbidium hybridum differ extremely significantly; 2) 2,4-D is a necessary factor to induce callus, and the frequency of callus formation increases with the increase of concentrations, but the explants will turn brown and die when the concentration is above 2.0mg/L; 3) KT is the key factor to induce formation of somatic embryogenesis, and its effects are restrained by 2,4-D and accelerated by a low concentration of NAA; 4) the frequency of somatic embryogenesis formation is also related to the genotype, but its dependence on the genotype has clustering and scattering characteristics.
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