Effects of pre-chilling on germination of Robinia pseudoacacia under salt stress
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摘要: 先将刺槐种子在始温80℃下浸泡24h,取吸胀的种子用低温3~5℃处理5、10、15d,置于不同盐分梯度下做发芽试验,观察种子的萌发能力.研究结果表明:在低于0.5%盐浓度下,低温处理5d能显著提高种子的发芽率和发芽势,发芽指数与活力指数明显高于对照;低温处理10d次之;低温处理15d对种子有伤害作用.低温处理组可溶性蛋白含量、超氧物岐化酶(SOD)的活性普遍提高;0.2%盐浓度下处理组过氧化物酶(POD)高于对照组,0.2%以上处理组显著低于对照;各种盐浓度下低温处理组过氧化氢酶(CAT)的活性均显著高于对照.该研究结果表明种子萌发前经5d低温(3~5℃)预处理能有效提高盐胁迫下刺槐种子的萌发能力.Abstract: The imbibed black locust seeds were treated with pre-chilling (3-5℃) for different days (5,10 and 15 d) and their germination capacity under different concentrations of NaCl (0,0.1%,0.2%,0.3%,0.4% and 0.5%) was analyzed.Results show that the germination rate and germination energy of black locust seeds treated with NaCl for 5d increased significantly, and their germination and vigor index were also higher than those of control if NaCl concentration was not more than 0.5%; followed by the seeds treated with pre-chilling for 10 d; the seeds would be injured with a pre-chilling time more than 15 d. During the gemination the activities of superoxide dismutase (SOD) and catalase (CAT) of treated seeds all increased; the activity of peroxidase (POD) was higher (NaCl concentration less than 0.2%) or less (NaCl concentration more than 0.2%) than those of control. These indicate that pre-chilling treatment could improve both the salt resistance and the seedling growth of black locust seeds during the germination.
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Keywords:
- black locust seed /
- pre-chilling treatment /
- salt resistance /
- germination
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