Abstract: The photosynthetic physiological characteristics of potcultured Camellia nitidissima seedlings were studied with a Li-6400 (LI-COR. Lincoln, Nebraska, USA) portable photosynthesis system. The results show that under open conditions without shade, the diurnal changes in the photosynthetic rate (Pn) showed a curve with two peaks and a clear phenomenon of midday depression in photosynthesis. The maximum photosynthetic rate (3.38 μmol/(m 2·s) appeared at 8:00 AM during the summer. The light saturation (LSP) and light compensation points (LCP) were 476.46 μmol/(m2·s) and 6.13 μmol/(m2·s), respectively. They were very low, indicating that these seedlings required shade condition. Under control condition (temperature 30℃, light 1 000 μmol/(m2·s) and humidity 50%-60%), the photosynthetic capacity was 12.58-13.50 μmol/(m 2·s) and the photosynthetic rate increased linearly when the concentration of CO 2 increased from 25 μmol/mol to 1 000 μmol/mol. The CO2 saturation point (CSP) was 1 50000 μmol/mol, the compensation point (Г) 99.48 μmol/mol and the maximum carboxylation rate (CE) 0.011 8 The seedlings tended to require heat, their photosynthetic rate reached its highest value at 35℃ and had a certain capacity to adapt to lowtemperatures, but could not tolerate 40℃. The seedlings were sensitive to drought stress and even a slight water deficit could depress their photosynthesis. 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