Abstract: The carbon content of Populus ×euramericana ‘Zhonglin46’ tissues from 8,10,13 and 16 years old at Yuncheng County in Shangdong Province were measured using the oxidation of K2Cr2O7-H2SO4 method. The main results show that the average carbon content of leaves, branches, trunks and roots was 55.13%, 54.65%, 50.05% and 45.40%, respectively. According to the average values, the sequence of carbon content in different organs from the maximum to the minimum was leaves, branches, trunks and roots. Additionally, in different layers of a tree, the sequence of carbon content in leaves from the maximum to the minimum was: the lower strata, the middle strata, the upper strata. The sequence of carbon content in branches was: the middle strata, the upper strata, the lower strata. The sequence of carbon content in trunks or roots was: the upper strata, the middle strata, the lower strata. Among different ages, the carbon content of organs increased when trees were growing. But the difference among layers or ages was not statistically significant (P0.05). The weighted average value of organ carbon content in P.×euramericana ‘Zhonglin46’ was 51.37% when the weight coefficient was the proportion of individual biomass to the sum of biomass. 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