Abstract: Based on a field investigation of the diseaseresistance of poplar, six varieties of poplar callus were selected and inoculated by the canker pathogen Dothiorella gregaria Sacc. The disease resistance of poplar callus was tested and the activities of four antioxidases related to disease resistance, i.e. POD, SOD, CAT and APX, were measured in order to evaluate the disease resistance of poplar. After infection by the canker pathogen, the activities of the four enzymes in the poplar callus all increased, of which the activity of POD increased significantly, while that of APX varied but slightly. The increases varied among the different poplar varieties. Compared with the control group, the varieties with stronger resistance in the field had higher enzyme activities, and the peaks of enzymes appeared earlier. 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