Abstract: This study established an effective method to purify microcystin-LR(MCLR) from laboratory-cultured Microcystis aeruginosa by a suitable combination of liquid\|liquid extraction and normal-pressure column chromatography technologies. First, the crude extraction of MCLR was processed by n-hexane extraction to remove lipid substances and solid phase extraction (SPE) to realize preliminary purification. Then the Sephadex LH-20 size exclusion chromatography was used to remove pigments and other impurities. The elution from LH-20 was collected by tubes and the absorbance was detected at 238 nm. We discovered that the second curve contained MCLR; its purity reached 40.5%. Finally, the MCLR sample was purified by DEAE Sepharose CL-6B anion exchange column chromatography, in which the ultra-pure water was used as an initial buffer and 3 mmol/L NaCl was used to elute MCLR. The purity of MCLR reached more than 90%, with a final yield of 66.2% after DEAE. 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