Abstract: We analyzed the composition and amount of anthocyanins in blue flowers of Senecio cruentus at different stages of flower development. High performance liquid chromatography with a photodiode array detector (HPLC-PAD) and HPLC-electrospray ionization-mass spectrometry (HPLC-ESI-MS) methods were employed for qualitative and quantitative analyses of anthocyanins. The results show that there are more than three kinds of delphinidins in the blue flowers of S. cruentus. The amount of anthocyanins in blue flowers of S. cruentus quickly increased before the 4th flower stage. The amount of anthocyanin and flower stages were positively correlated，but a negative linear relationship was found after the 4th flower stage. The results indicate that the variation of anthocyanins in blue petals of S. cruentus is a mechanism adapted to pollination and complies with the rule of using nutrition in perennial herbage. 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