Abstract: The ultrastructural changes of mesophyll cells in two Robinia pseudoacacia hybrids, i.e. tetraploid R. pseudoacacia and diploid R. pseudoacacia, were observed under NaCl stress. The results showed that: 1) before salt stress, the chloroplasts of two R. pseudoacacia were morphologically integrated. After 10 days of stress, the chloroplast of diploid R. pseudoacacia began deformed, with ambiguity in membrane and disorganization in grana lamell. After 20 days, the chloroplast showed further swelling and disarrangement, with a clear degradation of membrane. For tetraploid R. pseudoacacia, the structure of chloroplast changed little after 10 and 20 days of stress; 2) Before salt stress, the mitochondria of two R. pseudoacacia were morphologically integrated. After 10 days of stress, the cavitas and ambiguous crista were observed in the mitochondria of diploid R.pseudoacacia, which appeared ambiguity in membrane and lost integrity. After 20 days, most of mitochondria lost complete membrane and showed compatible with cytoplasm; no clear cristae but many cavitas were observed. The mitochondria of tetraploid R. pseudoacacia changed little with salt stress; 3) Before salt stress, the mesophyll cell of two R. pseudoacacia showed loose arrangement and complete structure. The chloroplast distributed at the margin of the cell wall. After 10 days, parts of chloroplast in diploid R. pseudoacacia moved to the middle of cell. After 20 days, most of chloroplast located in the middle of cell and showed random distribution; cell membrane degraded partly and became ambiguity. The shape of mesophyll cell of tetraploid R. pseudoacacia changed little under salt stress. The above results indicate that tetraploid R. pseudoacacia has anatomic adaptation to the salt stress. 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