Abstract: A HPLC chromatographic fingerprint system for the cultivated Taxus chinensis var. mairei was established based on the chromatographic fingerprint characteristics of leaves and branches from 11 batches of the 6-year cultivars in the Chongqing region. The optimum chromatography conditions are as follows: a SHIMADZU C18 column (250 mm×4.6 μm, 5 μm), a mobile phase of acetonitrile and water as gradient elution, a detective wave-length of 227 nm and a flow rate of 0.8 mL/min. Under these conditions, a total of 17 shared peaks were detected, of which No.9 was selected as the reference peak. The average relative standard deviation (RSD) of both the relative retention time and the relative area of shared peaks was lower than 2.0%. The unshared peak area occupied 4.1%--8.3% of the total, which conforms to the requirement of chromatographic fingerprint regulations (lower than 10%). Correlation analysis showed that the similarity of the shared peak was ≥93%, indicating that this chromatographic fingerprint system is stable, accurate and reproducible and can be recommended for identification of cultivated T. chinensis var. mairei. 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