Abstract: The factors affecting in vitro culture and plant regeneration of Lonicera edulis were optimized by orthogonal design and analysis of variance using sprouting buds as explants. Tissue cultured seedlings were obtained on culture media with different concentrations of hormones. The results show that the optimum compositions for primary culture, subculture and rooting culture are MS+6-BA 1.0 mg/L + IBA 0.2 mg/L, MS+6-BA 0.5 mg/L +IBA 0.3 mg/L +KT 1.5 mg/L and 1/4MS+IBA 1.5 mg/L. The differentiation rate, proliferation coefficient and rooting rate on these media were up to 100%, 8.7 and 96.7%, respectively. The survival rate of the rooted plantlets could reach 97.8% after acclimatization and transplantation. The combination of 6-BA, IBA and KT is effective in shoot proliferation of L. edulis in the subculture. This micropropagation technology could be applied in mass propagation. 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