川金丝猴粪样内皮质醇激素保存时效的研究
Effective storage period of cortisol in fresh feces of the Sichuan snub-nosed monkey.
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摘要: 野生动物新鲜粪便内激素的测定是探查动物生理状态的非损伤性方法，但新鲜粪便的保存时效因物种等而异。因此，掌握目标物种的新鲜粪便内激素的保存时效是应用该方法的前提。采取95％乙醇（以下简称乙醇）、低温(（4±1)℃，以下简称低温）方法保存川金丝猴的新鲜粪样，设保存0（标准参照）、5、6、7、8、10、20、30 d 8个处理。在各处理的保存时间段到期时,运用RIA法测定粪样内皮质醇激素的含量。结果显示:低温条件下,雌性川金丝猴粪样内皮质醇含量在保存6 d时的平均值显著低于标准参照值（P＜0.05），因此可以稳定保存5 d；雄性川金丝猴粪样内皮质醇含量在保存6 d时的平均值显著高于标准参照值（P＜0.05），因此可以稳定保存5 d。针对川金丝猴生存环境的特点,并据此设定该物种新鲜粪便保存方法和低温条件，所获得的测定结果是该物种野外粪样采集及保存的重要依据，可应用于该物种的野外相关研究。Abstract: The determination of hormones in fresh feces is a non-destructive method to detect the physiological status of wildlife; however, the effective storage period of fresh feces varies among different animal species. Therefore, understanding the effective storage period of physiological hormones in fresh feces for targeted species is a precondition to use this method. To determine the effective storage period of cortisol in fresh feces of the Sichuan snub-nosed monkey (Rhinopithecus roxellanae), we analyzed the content of cortisol with a RIA method under storage conditions of 95% ethanol at low temperature (4±1)℃ after eight storage periods, i.e. 0 (control), 5, 6, 7, 8, 10, 20 and 30 days. The results showed that after a storage period of six days, the content of female fecal cortisol was significantly lower than that of control (P0.05), while those of male fecal cortisol was significantly larger than control (P0.05), indicating that the safe storage period for cortisol is 5 days or less. The experimental conditions were established according to the habitat of species, so the results can be an important reference for the collection and storage of fresh feces and a basis for related researches.
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Keywords:
- Sichuan snub-nosed monkey /
- fresh feces /
- cortisol /
- effective storage period
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期刊类型引用(6)
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6. 方露，常亮，郭文静，陈勇平，王正. 高温处理对木塑复合胶合板性能的影响. 木材工业. 2014(04): 5-8 . 百度学术
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