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    李慧, 张茜, 付文锋, 张金凤. 离体条件下油松成熟合子胚不定芽的形成[J]. 北京林业大学学报, 2010, 32(5): 111-115.
    引用本文: 李慧, 张茜, 付文锋, 张金凤. 离体条件下油松成熟合子胚不定芽的形成[J]. 北京林业大学学报, 2010, 32(5): 111-115.
    LI Hui, ZHANG Qian, FU Wen-feng, ZHANG Jin-feng. Adventitious bud formation in vitro from mature zygotic embryos of Pinus tabulaeformis Carr.[J]. Journal of Beijing Forestry University, 2010, 32(5): 111-115.
    Citation: LI Hui, ZHANG Qian, FU Wen-feng, ZHANG Jin-feng. Adventitious bud formation in vitro from mature zygotic embryos of Pinus tabulaeformis Carr.[J]. Journal of Beijing Forestry University, 2010, 32(5): 111-115.

    离体条件下油松成熟合子胚不定芽的形成

    Adventitious bud formation in vitro from mature zygotic embryos of Pinus tabulaeformis Carr.

    • 摘要: 为确定油松成熟胚离体培养诱导不定芽的最适条件,以油松成熟胚为外植体材料,分别接种在TE、DCR和MS 3种基本培养基上以确定最适培养基;随后按照U*6(64)均匀设计表,在TE培养基中添加不同浓度梯度的植物生长激素6--BA、NAA、KT,建立试验组合以确定最佳激素配比;基于上述实验,进一步探讨了蔗糖浓度对不定芽诱导的影响及6--BA对不定芽生长的影响。结果表明:基本培养基对于不定芽的诱导起重要作用,在TE培养基上不定芽诱导率最高,DCR次之,MS最差;运用Uniform Design Version 3.00均匀设计软件,通过数据处理、回归分析及试验优化,得到激素最佳组合为:6--BA 6 mg/L +NAA 0.1 mg/L,KT对试验没有显著影响;蔗糖浓度为4.0%时不定芽诱导率最高;培养基中降低6--BA的浓度有利于不定芽的生长,在TE+6--BA 0.5 mg/L + NAA 0.1 mg/L的培养基中,丛生芽平均长度最长。

       

      Abstract: In order to determine the optimal conditions of inducing adventitious buds from mature embryos of Pinus tabulaeformis Carr., the mature embryos used as explants were respectively inoculated on TE, DCR and MS media to induce adventitious buds to find the best basal medium. According to uniform design U*6(64), the adventitious bud induction was analyzed by adding different concentrations of 6-BA,NAA and KT on TE basal medium. Based on the above experiments, further studies of the effects of sucrose concentration on the induction of adventitious buds and those of 6-BA on the growth of adventitious buds were carried out. The results showed that the basal medium played a determinative role in initiating adventitious buds. TE was the best, followed by DCR, and MS was the worst. Data processing, regression analysis and test optimization were completed using the Uniform Design Version 3.00 software. The optimal combination of growth regulators choice was 6-BA 6 mg/L+NAA 0.1 mg/L, and there was a lack of significant difference for KT. When the sucrose concentration was 4.0%, there was the highest rate of adventitious buds. Lower concentration of 6-BA was in favor of elongation of adventitious buds. The average length of adventitious buds was longest when using TE medium supplemented with 0.5 mg/L 6-BA and 0.1 mg/L NAA.

       

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