Abstract: The optimal tissue culture conditions for induction, differentiation, proliferation and rooting of adventitious buds of Pueraria lobata Ohwi were studied through in vitro culture and rapid propagation technologies. The results showed that using stem segments with axillary buds from elite parent trees of P. lobata as explants, the survival rate could reach 50% after 25－30 min of sterilization with 2.2% NaClO. The most optimal medium for induction of adventitious buds was MS + NAA 0.1 mg/L + 6-BA 0.5 mg/L, and the induction rate reached 88.89%. The most optimal medium for differentiation and subculture of the adventitious buds was MS + NAA 0.15 mg/L + 6-BA 0.3 mg/L + 2,4-D 0.2 mg/L; the differentiation rate reached 83.33%, and the multiples of proliferation rate ranged from 6 to 7. The most optimal rooting medium was 1/2 MS supplemented with NAA 0.5 mg/L, and the rooting rate could reach 100%. Overall, a tissue culture system and a relatively good technological system for rapid propagation of P. lobata were established.