Abstract: In order to achieve the high value-added-value utilization of papermulberry (Broussonetia papyriera)leaves, we selected four factors which had effect on extraction rate of chlorophyll, i.e. extraction temperature, ultrasonic extraction time, ultrasonic power and ratio of liquid to material. Through single factor experiment and response surface analysis (RSA) with four factors three levels, the extraction conditions of ultrasonic wave assisted extraction for chlorophyll from papermulberry leaves were optimized. The results showed that the optimum extraction conditions were an extraction temperature of 60℃, ultrasonic extraction time 50 min, ultrasonic power 500 W and ratio of liquid to material 10 mL/g. Under the optimum extraction conditions, the extraction rate of the chlorophyll could reach 1.277%. Compared to conventional grinding extraction and solvent extraction, ultrasonic wave assisted extraction for chlorophyll from papermulberry leaves greatly saved extraction time and increased extraction rate of active principle, so this method has a good prospect of application.