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    汪春蕾, 赵敏, 杨洪一, 崔岱宗, 李泰仑, 李德斌, 张宁. 一株产漆酶细菌的分离、鉴定及对造纸黑液处理的初步研究[J]. 北京林业大学学报, 2011, 33(3): 81-85.
    引用本文: 汪春蕾, 赵敏, 杨洪一, 崔岱宗, 李泰仑, 李德斌, 张宁. 一株产漆酶细菌的分离、鉴定及对造纸黑液处理的初步研究[J]. 北京林业大学学报, 2011, 33(3): 81-85.
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    WANG Chun-lei, ZHAO Min, YANG Hong-yi, CUI Dai-zong, LI Tai-lun, LI De-bin, ZHANG Ning. Isolation and identification of a bacterium strain exhibiting laccase activity and its treatment to papermaking black liquid[J]. Journal of Beijing Forestry University, 2011, 33(3): 81-85.
    Citation: WANG Chun-lei, ZHAO Min, YANG Hong-yi, CUI Dai-zong, LI Tai-lun, LI De-bin, ZHANG Ning. Isolation and identification of a bacterium strain exhibiting laccase activity and its treatment to papermaking black liquid[J]. Journal of Beijing Forestry University, 2011, 33(3): 81-85.
    shu

    一株产漆酶细菌的分离、鉴定及对造纸黑液处理的初步研究

    Isolation and identification of a bacterium strain exhibiting laccase activity and its treatment to papermaking black liquid

      摘要
    • 摘要: 利用铜离子作为筛选剂，从凉水国家级自然保护区的土样中分离纯化出一株具有高漆酶活性的细菌。通过形态学观察、生理生化特性测定及16S rDNA序列分析，鉴定其为枯草芽孢杆菌(Bacillus subtilis)，命名为B. subtilis WD23。根据GenBank上公布的Bacillus subtilis cotA基因序列设计引物，克隆B. subtilis WD23的cotA基因，并采用pET-22b(+)/E.coli BL21(DE3)系统进行诱导表达，测定工程菌株发酵液的粗提液的漆酶活性达到1 700 U/mL，表明该菌株漆酶活性来源其芽孢。用固定化的B. subtilis WD23芽孢处理造纸黑液，处理7 d色度下降24.9%，化学需氧量(COD)下降31.2%。

       

      Abstract: A novel bacterium strain exhibiting high laccase activity was separated and purified from the forest soil of the Liangshui National Nature Reserve in Heilongjiang Province with Cu 2+ as the enrichment culture reagent. The strain was identified as Bacillus subtilis WD23 by morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis. The cotA gene was cloned by primers designed according to B. subtilis cotA gene sequence on the GenBank. The cotA gene was inducibly expressed by the system of pET22b(+)/E. coli BL21(DE3). The laccase activity of the culture extraction was 1 700 U/mL. The result suggests that the laccase activity is derived from the spore coat protein. The immobilized spore laccase could decolorize black pulping liquid with a decoloration rate of 24.9% and a COD reduction by 31.2% after seven days of treatment.

       

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