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    史军娜, 刘美芹, 师静, 王艳萍, 智冠华, 陈玉珍, 卢存福. 沙冬青GATA型锌指蛋白基因序列及表达分析[J]. 北京林业大学学报, 2011, 33(3): 21-25.
    引用本文: 史军娜, 刘美芹, 师静, 王艳萍, 智冠华, 陈玉珍, 卢存福. 沙冬青GATA型锌指蛋白基因序列及表达分析[J]. 北京林业大学学报, 2011, 33(3): 21-25.
    SHI Jun-na, LIU Mei-qin, SHI Jing, WANG Yan-ping, ZHI Guan-hua, CHEN Yu-zhen, LU Cun-fu. Sequence analysis and expression pattern of AmZFPG encoding a GATA type zinc finger protein in Ammopiptanthus mongolicus.[J]. Journal of Beijing Forestry University, 2011, 33(3): 21-25.
    Citation: SHI Jun-na, LIU Mei-qin, SHI Jing, WANG Yan-ping, ZHI Guan-hua, CHEN Yu-zhen, LU Cun-fu. Sequence analysis and expression pattern of AmZFPG encoding a GATA type zinc finger protein in Ammopiptanthus mongolicus.[J]. Journal of Beijing Forestry University, 2011, 33(3): 21-25.

    沙冬青GATA型锌指蛋白基因序列及表达分析

    Sequence analysis and expression pattern of AmZFPG encoding a GATA type zinc finger protein in Ammopiptanthus mongolicus.

    • 摘要: 根据沙冬青低温干旱诱导cDNA文库表达序列标签中锌指蛋白基因序列设计特异引物,采用PCR技术扩增锌指蛋白基因AmZFPG的cDNA全长。序列分析表明,cDNA全长669 bp,推测编码223 个氨基酸残基、分子量约25 kD的蛋白质。氨基酸保守性分析及同类锌指结构比对表明AmZFPG属于GATA结合蛋白家族,且具有高度的保守性。系统发生分析表明,其与紫花苜蓿富含脯氨酸的细胞壁蛋白进化亲缘关系最近。半定量 RT-PCR分析显示,AmZFPG基因是受低温和干旱诱导表达上调的基因,初步得出AmZFPG基因与沙冬青低温和干旱胁迫响应相关。

       

      Abstract: We designed special primers according to the zinc finger protein gene sequence in expressed sequence tags from a cold and droughttreated Ammopiptanthus mongolicus cDNA library and amplified cDNA of AmZFPG by PCR. The cDNA of AmZFPG is comprised of 669 base pairs which encode a zinc finger protein with 273 amino acids and a molecular weight of 25 kD. Bioinformatic analysis indicates that AmZFPG belongs to GATAbinding protein family and shares higher homology with given zinc finger proteins which are involved in stress resistance in other plant species. Phylogenetic analysis indicates that AmZFPG is most closely related to Medicago sativa prolinerich cell wall protein.Semiquantitative RTPCR analysis shows that the expression of AmZFPG is constitutive and upregulated under cold and drought stresses and that AmZFPG is related to response to drought and chilling stresses.

       

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