不同NH+4/NO-3配比作用下红松幼苗的氮吸收和同化特征
Nitrogen absorption and assimilation characteristics of Pinus koraiensis seedings in different NH+4/NO-3 ratios
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摘要: 采用营养液培养法，研究不同NH+4/NO-3配比供氮模式下红松幼苗的氮素吸收水平、主要氮同化酶活性及苗木生长水平。结果表明：红松幼苗在NH+4占优势的营养条件下吸收氮素能力较强、总生物量较大。苗木根系中硝酸还原酶（NR）活性较强，营养液中的NO-3对NR活性有明显的促进作用，且随其中供NO-3比例的增加而增强。谷氨酰胺合成酶（GS）活性和谷氨酸脱氢酶（GDH）活性则可能因外源NH+4的诱导作用而增加；与根系相比，针叶中GS活性较强，且NH+4优势处理苗木的酶活性较强。GDH活性在苗木根系和针叶中均较强，且在供NH+4优势时酶活性较强。
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关键词:
- 红松幼苗 /
- NH+4/NO-3配比 /
- 氮吸收 /
- 氮同化酶 /
- 生物量
Abstract: A solution cultivation experiment with Pinus koraiensis seedlings was conducted to study the effect of different NH+4/NO-3 ratio on nitrogen absorption，nitrogen assimilation enzyme and growth. The results showed that more nitrogen was absorbed by the seedlings in high NH+4/NO-3 ratio solution, whose total biomass was more than the others. Nitrate reductase (NR) activity was higher in the roots than the leaves, NO-3 promoted NR activity, which responsed to increased NO-3 availability of the solution. Glutamine synthetase (GS) activity and glutamate dehydrogenase (GDH) activity was improved by the induction of exogenous NH+4. GS activity was higher in the leaves than the roots, and which was higher in the seedlings of high NH+4/NO-3 ratio solution. GDH activity of the roots and the leaves was high, and which was higher in the seedlings of high NH+4/NO-3 ratio solution. -
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