3种柳树叶片PSⅡ叶绿素荧光参数对Cd2+胁迫的光响应

钱永强; 周晓星; 韩蕾; 孙振元; 巨关升

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3种柳树叶片PSⅡ叶绿素荧光参数对Cd2+胁迫的光响应

钱永强, 周晓星, 韩蕾, 孙振元, 巨关升

文章导航 > 北京林业大学学报 > 2011 > 33(6): 8-14

钱永强, 周晓星, 韩蕾, 孙振元, 巨关升. 3种柳树叶片PSⅡ叶绿素荧光参数对Cd2+胁迫的光响应[J]. 北京林业大学学报, 2011, 33(6): 8-14.

引用本文:

钱永强, 周晓星, 韩蕾, 孙振元, 巨关升. 3种柳树叶片PSⅡ叶绿素荧光参数对Cd2+胁迫的光响应[J]. 北京林业大学学报, 2011, 33(6): 8-14.

QIAN Yong-qiang, ZHOU Xiao-xing, HAN Lei, SUN Zhen-yuan, JU Guan-sheng. Rapid light-response curves of PSⅡ chlorophyll fluorescence parameters in the leaves of Salix babylonica, Salix ‘J172’ and Salix leucopithecia to Cd2+ stress[J]. Journal of Beijing Forestry University, 2011, 33(6): 8-14.

Citation:

QIAN Yong-qiang, ZHOU Xiao-xing, HAN Lei, SUN Zhen-yuan, JU Guan-sheng. Rapid light-response curves of PSⅡ chlorophyll fluorescence parameters in the leaves of Salix babylonica, Salix ‘J172’ and Salix leucopithecia to Cd2+ stress[J]. Journal of Beijing Forestry University, 2011, 33(6): 8-14.

3种柳树叶片PSⅡ叶绿素荧光参数对Cd2+胁迫的光响应

1.
中国林业科学研究院林业研究所，林木遗传育种国家重点实验室，国家林业局林木培育重点实验室

Rapid light-response curves of PSⅡ chlorophyll fluorescence parameters in the leaves of Salix babylonica, Salix ‘J172’ and Salix leucopithecia to Cd2+ stress

1.
Research Institute of Forestry, State Key Laboratory of Forest Genetics and Tree Breeding，Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Chinese Academy of Forestry, Beijing, 100091, P. R. China.

摘要: 以盆栽垂柳、苏柳‘J172’及银芽柳为材料，利用MINIIMAGINGPAM荧光成像测定系统，测定了Cd2+胁迫下柳树叶片叶绿素荧光淬灭特性及各荧光参数的光响应曲线。结果表明，Cd 2+胁迫下柳树叶片初始荧光(F0)与最大荧光(Fm)均显著增大，200 mg/L Cd 2+处理2周时，F0与Fm均达到峰值。并分析了200 mg/L Cd 2+胁迫下各柳树叶片叶绿素荧光参数的光响应，结果显示：PSⅡ最大光化学效率 (Fv/Fm)、PSⅡ实际光量子效率 (PSⅡ)、光化学淬灭系数 (qP) 随光化光强度的增加呈下降趋势；同光强作用下，受Cd 2+ 胁迫的3种柳树叶片PSⅡ与 qP 均显著降低，但柳树种间存在显著差异；PSⅡ调节性能量耗散的量子产额YNPQ、非光化学淬灭系数 (qN) 与表观电子传递速率 (ETR) 则随着光化光强度增加呈上升趋势；同光强作用下，Cd2+胁迫使YNPQ、qN 与ETR显著提高；Cd 2+胁迫下，柳树叶片PSⅡ非调节性能量耗散的量子产额YNO随光强的增加无明显变化，均稳定在较低水平，但显著高于对照。推断柳树通过增加非光化学淬灭形式耗散过剩能量或提高光化学转化与光能利用效率，调节PSⅡ反应中心开放程度与活性，表现出对Cd 2+胁迫的耐性。
- 柳树 /
- 叶绿素荧光 /
- 镉离子胁迫 /
- 快速光响应曲线
Abstract: The effects of Cd 2+ on rapid lightresponse curves of photochemical and nonphotochemical chloropyll fluorescence quenching parameters were investigated under different cadmium treatments based on the determination of chlorophyll fluorescence parameters. Dark fluorescence yield (F0) and maximal fluorescence yield (Fm) of Salix babylonica, S. ‘J172’ and S. leucopithecia were investigated under different concentrations of Cd2+ solutions (0,50,100,200,400 mg/L) by a MINIIMAGINGPAM chlorophyll fluorometer. The results showed that F0 and Fm significantly increased compared with control, and the two parameters reached the maximum under the stress of 200 mg/L Cd 2+ for 2 weeks. The rapid lightresponse curves of chlorophyll fluorescence parameters of the three willow (Salix spp.) trees were also measured at various light intensities. The parameters of maximal PSⅡ quantum yield (Fv/Fm), effective PSⅡ quantum yield (PSⅡ) and coefficient of photochemical quenching (qP) decreased according to the increase of light intensity. The PSⅡ and qP declined significantly under Cd 2+ stress at the same light intensity compared with control. The parameters of quantum yield of regulated energy dissipation (YNPQ), coefficient of nonphotochemical quenching (qN), and electron transport rate (ETR) increased with elevation of light intensity. The YNPQ, qN, and ETR increased sharply under Cd 2+ stress at same light intensity. No significant change was found for quantum yield of nonregulated energy dissipation(YNO) of the three willow trees with the aggrandizement of light intensity. The results indicate that S. leucopithecia shows torelence to Cd 2+ by reversible inactivation of PSⅡ reaction center.
- Salix spp. /
- chlorophyll fluorescence /
- cadmiumion stress /
- rapid lightresponse curve

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3种柳树叶片PSⅡ叶绿素荧光参数对Cd2+胁迫的光响应

1. 中国林业科学研究院林业研究所，林木遗传育种国家重点实验室，国家林业局林木培育重点实验室

收稿日期: 1900-01-01
修回日期: 1900-01-01
刊出日期: 2011-11-30

关键词:

柳树 /

叶绿素荧光 /

镉离子胁迫 /

快速光响应曲线

摘要: 以盆栽垂柳、苏柳‘J172’及银芽柳为材料，利用MINIIMAGINGPAM荧光成像测定系统，测定了Cd2+胁迫下柳树叶片叶绿素荧光淬灭特性及各荧光参数的光响应曲线。结果表明，Cd 2+胁迫下柳树叶片初始荧光(F0)与最大荧光(Fm)均显著增大，200 mg/L Cd 2+处理2周时，F0与Fm均达到峰值。并分析了200 mg/L Cd 2+胁迫下各柳树叶片叶绿素荧光参数的光响应，结果显示：PSⅡ最大光化学效率 (Fv/Fm)、PSⅡ实际光量子效率 (PSⅡ)、光化学淬灭系数 (qP) 随光化光强度的增加呈下降趋势；同光强作用下，受Cd 2+ 胁迫的3种柳树叶片PSⅡ与 qP 均显著降低，但柳树种间存在显著差异；PSⅡ调节性能量耗散的量子产额YNPQ、非光化学淬灭系数 (qN) 与表观电子传递速率 (ETR) 则随着光化光强度增加呈上升趋势；同光强作用下，Cd2+胁迫使YNPQ、qN 与ETR显著提高；Cd 2+胁迫下，柳树叶片PSⅡ非调节性能量耗散的量子产额YNO随光强的增加无明显变化，均稳定在较低水平，但显著高于对照。推断柳树通过增加非光化学淬灭形式耗散过剩能量或提高光化学转化与光能利用效率，调节PSⅡ反应中心开放程度与活性，表现出对Cd 2+胁迫的耐性。

English Abstract

Rapid light-response curves of PSⅡ chlorophyll fluorescence parameters in the leaves of Salix babylonica, Salix ‘J172’ and Salix leucopithecia to Cd2+ stress

1. Research Institute of Forestry, State Key Laboratory of Forest Genetics and Tree Breeding，Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Chinese Academy of Forestry, Beijing, 100091, P. R. China.

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2011-11-30

Keywords:

Abstract: The effects of Cd 2+ on rapid lightresponse curves of photochemical and nonphotochemical chloropyll fluorescence quenching parameters were investigated under different cadmium treatments based on the determination of chlorophyll fluorescence parameters. Dark fluorescence yield (F0) and maximal fluorescence yield (Fm) of Salix babylonica, S. ‘J172’ and S. leucopithecia were investigated under different concentrations of Cd2+ solutions (0,50,100,200,400 mg/L) by a MINIIMAGINGPAM chlorophyll fluorometer. The results showed that F0 and Fm significantly increased compared with control, and the two parameters reached the maximum under the stress of 200 mg/L Cd 2+ for 2 weeks. The rapid lightresponse curves of chlorophyll fluorescence parameters of the three willow (Salix spp.) trees were also measured at various light intensities. The parameters of maximal PSⅡ quantum yield (Fv/Fm), effective PSⅡ quantum yield (PSⅡ) and coefficient of photochemical quenching (qP) decreased according to the increase of light intensity. The PSⅡ and qP declined significantly under Cd 2+ stress at the same light intensity compared with control. The parameters of quantum yield of regulated energy dissipation (YNPQ), coefficient of nonphotochemical quenching (qN), and electron transport rate (ETR) increased with elevation of light intensity. The YNPQ, qN, and ETR increased sharply under Cd 2+ stress at same light intensity. No significant change was found for quantum yield of nonregulated energy dissipation(YNO) of the three willow trees with the aggrandizement of light intensity. The results indicate that S. leucopithecia shows torelence to Cd 2+ by reversible inactivation of PSⅡ reaction center.

钱永强, 周晓星, 韩蕾, 孙振元, 巨关升. 3种柳树叶片PSⅡ叶绿素荧光参数对Cd2+胁迫的光响应[J]. 北京林业大学学报, 2011, 33(6): 8-14.

引用本文:

钱永强, 周晓星, 韩蕾, 孙振元, 巨关升. 3种柳树叶片PSⅡ叶绿素荧光参数对Cd2+胁迫的光响应[J]. 北京林业大学学报, 2011, 33(6): 8-14.

Citation:

QIAN Yong-qiang, ZHOU Xiao-xing, HAN Lei, SUN Zhen-yuan, JU Guan-sheng. Rapid light-response curves of PSⅡ chlorophyll fluorescence parameters in the leaves of Salix babylonica, Salix ‘J172’ and Salix leucopithecia to Cd2+ stress[J]. Journal of Beijing Forestry University, 2011, 33(6): 8-14.