利用ISSR标记探讨贵州地方樱桃种质玛瑙红的起源原因
Exploring the original causes of cherry cultivar Manaohong originated from Nayong County, Guizhou Province using ISSR markers.
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摘要: 为探讨玛瑙红的起源原因，利用ISSR标记对贵州省纳雍县地方樱桃种质玛瑙红、酸樱桃、黑珍珠和4种欧洲甜樱桃等种质的亲缘关系进行分析。结果表明：用15条稳定性强、条带清晰及多态性丰富的引物进行PCR扩增，共获得286个标记位点，其中多态性位点为249个，多态性比例为87.06%；采用NTSYS2.01软件计算，7种樱桃相似性系数为0.41~0.94，玛瑙红与酸樱桃的相似系数为0.57。玛瑙红和酸樱桃在分子水平上的遗传差异很大，该种质可能为酸樱桃的实生变异种质。Abstract: In this paper, genetic relationship among local cherry cultivar Manaohong originated from Nayong County of Guizhou Province, as well as: sour cherry, black pearl and four sweet cherry cultivars were investigated using ISSR markers. The purpose was to investigate the causes of variation on Manaohong. A total of 286 markers were scored from 15 primers, which were demonstrated highly reproducible, clear and polymorphic bands. Among the obtained markers, 249 were polymorphic, accounting for 87.06% of the total. As analyzed by NTSYS2.01, the genetic similarity among the seven accessions ranged from 0.41 to 0.94, and the genetic similarity between Manaohong and sour cherry was 0.57Manaohong has significant genetic differences with sour cherry on molecular level, possibly it is a hybridization variation of sour cherry.
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Keywords:
- cherry /
- germplasm /
- Manaohong /
- Guizhou Province /
- ISSR
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期刊类型引用(4)
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