西藏核桃优良单株的FISH-AFLP分析

马庆国; 王滑; 潘刚; 裴东

西藏核桃优良单株的FISH-AFLP分析

马庆国¹,
王滑^1,2,
潘刚³,
裴东¹

1 中国林业科学研究院林业研究所，林木遗传育种国家重点实验室2 华中农业大学园艺林学学院3 西藏农牧学院西藏高原生态研究所

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出版历程
- 收稿日期: 1899-12-31
- 修回日期: 1899-12-31
- 发布日期: 2011-11-29

FISH-AFLP analysis of walnut advanced trees in Tibet, southwestern China.

1 Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing, 100091, P. R. China; 2 College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, P. R. China; 3 Research Institute of Plateau Ecology, Tibet Agriculture and Animal Husbandry College, Linzhi, 860000, P. R. China.

摘要

摘要: 为了深入了解西藏核桃种质资源的遗传背景和遗传多样性，采用FISHAFLP技术，筛选9 对EcoRⅠ+3和MseⅠ+3引物组合，对我国西藏核桃主产区加查和郎县的11个核桃(Juglans regia L.)优良单株进行基因组DNA水平检测。结果表明：在获得的1 103条可统计条带中，1 076 条呈多态性，多态性达97.67%；9对引物所检测到的不同位点的有效等位基因数(Ne)范围为1.00~1.99，平均为1.48±0.04；基因多样度(H)为0.00~0.49，平均为0.29±0.02；Shannon信息指数(I)为0.00~0.69，平均为0.44±0.02。本研究分析了西藏核桃优良单株的遗传多样性和亲缘关系，建立了它们的指纹图谱。
- 核桃 /
- 遗传多样性 /
- 亲缘关系 /
- AFLP /
- 指纹图谱
Abstract: In order to deeply understand the genetic background and the genetic diversity of Tibet walnut germplasm resources, FISHAFLP was conducted using 9 EcoRⅠ+3/MseⅠ+3 primer combinations to analyse 11 walnut (Juglans regia L.) advanced trees from Tibet at genomic DNA level. Totally 1 103 AFLP fragments were obtained, in which 1 076 were polymorphic and the average percentage of polymorphic bands (ppb) was 97.67%. The value of effective number of alleles (Ne) ranged from 1.00 to 1.99 with an average of 1.48±0.04; Neis gene diversity (H) ranged from 0.00 to 0.49 with an average of 0.29±0.02 and Shannons information index (I) ranged from 0.00 to 0.69 with an average of 0.44±0.02The genetic diversity and sibship among walnut advanced trees in Tibet were investigated and their fingerprintings were established in the research.
- Juglans regia L. /
- genetic diversity /
- sibship /
- AFLP /
- fingerprinting

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文章访问数: 2624
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西藏核桃优良单株的FISH-AFLP分析

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FISH-AFLP analysis of walnut advanced trees in Tibet, southwestern China.

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