Abstract: The rooting effects of donor plant age, plant growth regulators and their concentrations on stem cuttings from Russian seabuckthorn were investigated. The factors mentioned above were highly significant (P001) for all studied parameters and the rooting effects of stem cuttings form 1-year-old donor tree were better than from 2-year-old one. NAA was more effective than IBA and ABT2among plant growth regulators and the 200 mg/L concentration of plant growth regulator was the best. Overall, applying 200 mg/L concentration of NAA to treat 1-year-old donor tree cuttings for 30 min could achieve the best rooting, with the rooting rate, root number and average root length being 88.87%, 10.8 item/panicle and 10.47 cm, respectivly. Meanwhile, the interaction among the factors also had significant effects on rooting of softwood cuttings. The interactive effects of donor age×plant growth regulators were significant for percent rooting. The interactive effects of plant growth regulators×concentration and age×plant growth regulator concentration were highly significant for percent rate, root number and average root length. The interactive effects of donor tree age×the concentration of plant growth regulators×plant growth regulators were highly significant for rooting rate of H. rhamnoides.