解除紫椴种子休眠技术的研究
Seed treatments to overcome dormancy of Tilia amurensis.
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摘要: 研究了紫椴种子的休眠与萌发条件，主要对其解除休眠的方法进行了研究。试验设计包括3种处理：不同时间的浓H2SO4处理(10、30、60 min)，4种不同的层积方式(无处理，0~5 ℃、30 d，15~20 ℃、30 d，15~20 ℃、10 d→ 0~5 ℃、20 d)，不同浓度的GA3处理(0、100、300 mg/L)。每种处理设置4个重复。经上述处理的种子最终萌发率与平均萌发时间与对照相比显著提高；同时，15~20 ℃、10 d→ 0~5 ℃、20 d的变温层积比其他恒温层积更加有效；层积处理效果比浓H2SO4、GA3的处理效果更好。对紫椴种子处理的最佳组合是以浓H2SO4浸泡种子30 min，然后施以100 mg/L的GA3进行处理，再进行15~20 ℃、10 d→ 0~5 ℃、20 d的层积，发芽率高达90%。Abstract: The present research was conducted to study the dormancy breaking treatments in Tilia amurensis seed. An experiment was conducted with four replications and three treatments，including three different concentrated sulfuric acid treatments (10, 30 and 60 min), varying concentrations of GA3(0, 100 and 300 mg/L) and 4 levels of stratification (0; 0-5 ℃, 30 d; 15-20 ℃, 30 d and 15-20 ℃, 10 d→0-5 ℃, 20 d). The germination percentage and mean germination time of treated seeds were significantly enhanced by mentioned treatments compared with control. It was concluded that altering temperature stratification was more effective on studied dormancy breaking traits than others. Moreover, cold stratification was a better treatment on breaking seed dormancy of T. amurensis seeds than concentrated H2SO4and GA3 treatments. Applying concentrated sulfuric acid for 30 min and 100 mg/L concentration of GA3 and stratification(15-20 ℃, 10 d→05 ℃, 20 d) results in higher germination in T. amurensis dormant seeds, and germination percentage reaches 90%.
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Keywords:
- Tilia amurensis /
- seed /
- H2SO4 /
- GA3 /
- stratification /
- dormancy releasing
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