地锦属野生种及栽培品种的ISSR分析鉴定
ISSR analysis of Parthenocissus spp. and cultivars.
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摘要: 利用ISSR分子标记对9份地锦属植物材料进行基因组多态性分析，7条引物共扩增出74条带，其中多态带百分率达95.9%，所选引物能将全部供试材料区分开，并表现出很高的鉴定效率。利用NTSYSpc2.10e软件进行相似系数分析，9份地锦属植物材料的遗传相似系数在0.30～0.89之间，平均相似系数为0.517。通过扩增结果的UPGMA聚类，引入品种五叶地锦‘加引1号’与8个国内种质分开；8个国内种质又聚为5小叶型和非5小叶型两大类，与形态学的分类结果基本一致。研究表明，ISSR在地锦属种质资源分类和鉴定上具有一定的准确性，可以作为品种鉴定和新品种DUS测试的辅助工具。Abstract: Nine germplasms of Parthenocissus were analyzed for their genome polymorphism by ISSR. A total of 74 bands were amplified by 7 primers, of which 95.9% were polymorphic. All materials could be distinguished by the selected primers with high identification efficiency. The genetic similarity coefficients of the 9 germplasms varied among germplasms was 0.30 to 0.89 with an average of 0.517, computed with software NTSYSpc210e. Judging from the clustering dendrogram constructed by UPGMA method, an introduced cultivar P. quinquefolia ‘Jiayin 1’ was separated from 8 domestic species. The clustering dendrogram was constructed by UPGMA method. P. quinquefolia ‘Jiayin 1’ was separated from 8 domestic germplasms, and then 8 domestic germplasms were divided into two major groups with different leaf types. The domestic germplasms were further classified into two groups as also signified by their different leaf morphologies. In conclusion, ISSR analysis could be used for germplasm classification, variety identification and DUS testing in Parthenocissus spp. with reliable accuracy.
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Keywords:
- Parthenocissus /
- ISSR /
- genetic relationship /
- variety identification
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