Abstract: Nine germplasms of Parthenocissus were analyzed for their genome polymorphism by ISSR. A total of 74 bands were amplified by 7 primers, of which 95.9% were polymorphic. All materials could be distinguished by the selected primers with high identification efficiency. The genetic similarity coefficients of the 9 germplasms varied among germplasms was 0.30 to 0.89 with an average of 0.517, computed with software NTSYSpc210e. Judging from the clustering dendrogram constructed by UPGMA method, an introduced cultivar P. quinquefolia ‘Jiayin 1’ was separated from 8 domestic species. The clustering dendrogram was constructed by UPGMA method. P. quinquefolia ‘Jiayin 1’ was separated from 8 domestic germplasms, and then 8 domestic germplasms were divided into two major groups with different leaf types. The domestic germplasms were further classified into two groups as also signified by their different leaf morphologies. In conclusion, ISSR analysis could be used for germplasm classification, variety identification and DUS testing in Parthenocissus spp. with reliable accuracy.