Abstract: In order to improve the seedling survival rate and promote the growth of Picea pungens, which was introduced from the America, the suitable mycorrhizal fungi were selected and the technique of seedling cultivation with mycorrhizal fungi was established in 2007 The results showed that: 1) Schizophyllum commune and Pisolithus tinctorius（Pt） were the suitable mycorrhizal fungi. 2) The average heights of seedlings from different provenances had no significant differences after inoculating Pt, but the differences at varied inoculating time were extremely significant. 3) The suitable doses of Pt were varied among different provenances. For No.1 and No.3, the suitable inoculating dose was 1 g per plant, but for No.2 and No.4, it was 0.5 g per plant. The average seedling heights of four provenances after two months of growth were 3.31, 3.90, 3.84 and 3.54 cm respectively. 4) Inoculation with Pt at the transplanting time not only can improve survival rate, but also help maintain ornamental characteristics. The transplanting survival rate of all the treatments was above 85%, and that of the No.4 seedlings was the highest of 92.86%. The number of first class seedlings of all treatments increased compared with the control, and the first class seedling rate of No.3 seedlings was the highest of 56.67%.