沙冬青A20/AN1锌指蛋白基因序列及表达分析
Sequence analysis and expression pattern of AmSTZF encoding an A20/AN1 zinc finger protein in Ammopiptanthus mongolicus.
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摘要: A20/AN1锌指蛋白普遍存在于真核生物中,在胁迫响应引起的信号传导过程中发挥着重要作用。本文利用生物信息学和RT-PCR方法从低温干旱胁迫的沙冬青叶片组织中分离锌指蛋白基因AmSTZF,该基因编码一条含172个氨基酸残基的多肽,含A20和AN1两个锌指蛋白保守结构域,属A20/AN1锌指蛋白。生物信息学分析显示该蛋白定位于细胞质中,具有磷酸化位点,参与细胞转录调控。RTPCR分析表明:低温和干旱胁迫处理的沙冬青叶片中,AmSTZF的表达增强,初步推测该基因与沙冬青低温、干旱胁迫响应相关。
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关键词:
- 沙冬青 /
- A20/AN1锌指蛋白 /
- 序列分析 /
- 表达分析
Abstract: A20/AN1 zinc finger protein widely exists in eukaryote and plays a central role in signal transduction pathway involved in stress response.We cloned zinc finger gene AmSTZF from cold and droughttreated Ammopiptanthus mongolicus leaves by bioinformatics and RTPCR. The cDNA of AmSTZF encodes a polypeptide with 172 amino acids,which contains A20 and AN1 conserved domains and belongs to A20/AN1 zinc finger protein.Bioinformatics predicts that AmSTZF has phosphorylation sites, exists in cytoplasm and is involved in the regulation of gene transcription.RTPCR analysis shows that the expression of AmSTZF is upregulated under cold-and drought-stresses and that AmSTZF is related to the response to drought and chilling stresses. -
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期刊类型引用(4)
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2. 任美艳,杨晓茹,殷玉梅,郭慧琴,张艳霞,薛敏,王茅雁. 沙冬青RING型锌指蛋白基因AmRFP1的克隆与功能初步分析. 西北植物学报. 2017(12): 2317-2324 . 百度学术
3. 任美艳,王志林,郭慧琴,薛敏,殷玉梅,王茅雁. 沙冬青C2H2型锌指蛋白基因AmZFP1的克隆与表达分析. 华北农学报. 2017(02): 8-13 . 百度学术
4. 钟小娟. 植物低温逆境胁迫相关锌指蛋白研究进展. 北京农业. 2015(31): 207-208 . 百度学术
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