豚草提取液对小鼠肝脏CarE活性及蛋白质含量的影响
Effects of Ambrosia artemisiifolia extraction on CarE activity and protein content in mice liver.
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摘要: 采用超声法提取豚草活性物质，通过灌胃给药途径喂给小白鼠，以有机磷药剂敌敌畏(DDVP)和空白组作为对照，研究豚草提取液对哺乳动物肝脏羧酸酯酶(CarE)活性及蛋白质含量的影响。结果表明：豚草提取液对小鼠肝脏CarE活性有激活作用，在给药后6 h内酶比活力值上升显著，达到15.006 μmol/（min•mg）；随后逐渐降低，24 h时酶比活力值降至9.156 μmol/（min•mg），基本恢复至正常水平。DDVP对小鼠肝脏CarE活性产生明显抑制作用，给药后其CarE活性始终处于被抑制状态，显著低于空白组，且恢复缓慢。豚草提取液和DDVP均对小鼠肝脏蛋白质含量有抑制作用。作为杀虫抑菌制剂，豚草活性物质的安全性显著优于有机磷药剂。Abstract: The activity component in Ambrosia artemisiifolia was extracted with ultrasonic method and then the mice was fed through pouring the fluid into stomach. The organophosphorus pesticide DDVP and blank treatment were taken as control. The purpose of this research was to study the extracting solution of A. artemisiifolias effects on the activity of CarE and protein content of mammals. The results showed that extracting solution of A. artemisiifolia had the effect of activation on CarE. The enzyme activity value increased to 15.006 μmol/（min•mg） markedly during 6 hours after feeding pesticide. Then it decreased gradually and reached 9.156 μmol/（min•mg）, which was basically recovered to normal until 24 hours after feeding. Apparently, DDVP has the effect of inhibition on the activity of CarE of mice liver. The activity of CarE was in the state of inhibition after feeding pesticide and the value was lower than control and recovered slowly. Furthermore, both of extracting solution of A. artemisiifolia and DDVP have the effects of inhibition on protein content of mice liver. Therefore, as pesticide and antibacterial agent, the safety of the activity components of A. artemisiifolia is better than organophosphorus pesticide.
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