2个柽柳Prx基因的克隆及表达分析
Cloning and expression analysis of two novel Prxs genes from Tamarix hispida.
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摘要: 过氧化还原蛋白（Prxs）是植物体内重要的抗氧化酶。为深入研究Prx基因在柽柳非生物胁迫中的作用，从柽柳cDNA文库中获得了2条Prx的单一序列ThPrx1与ThPrx2，其中ThPrx1为部分序列，ThPrx2为全长序列。生物信息学分析表明，2条ThPrx与植物Prx同源基因的氨基酸序列具有较高的同源性，并且ThPrx1和ThPrx2分别属于Prx的PrxⅡ和2-Cys Prxs亚家族。通过实时定量PCR对这2个ThPrx基因在不同非生物胁迫（NaCl、PEG、CdCl2、低温及ABA）处理的柽柳根和叶中的表达模式分析显示， 2个ThPrx基因在胁迫下的表达模式不同：NaCl、PEG和ABA均能够诱导ThPrx1基因在根中的表达，而CdCl2和低温胁迫则抑制了该基因在根和叶中的表达；ThPrx2基因在低温处理的柽柳中的表达量下降，其他胁迫处理不同程度上影响了ThPrx2基因的表达。结果表明，ThPrx1与ThPrx2是2个新的Prx基因，推测其可能参与植物的逆境胁迫过程。Abstract: Peroxiredoxins (Prxs) are important antioxidant enzymes in nearly all plant tissues. To characterize their functions under abiotic stress, two Prx cDNA sequences were isolated from the cDNA library of Tamarix hispida, named ThPrx1 and ThPrx2 respectively. ThPrx1 is partial cDNA, while ThPrx2 is complete gene. Bioinformatics analysis showed that the two ThPrx sequences were highly homologous to other Prx genes from other plants, and ThPrx1 and ThPrx2 belong to the PrxⅡ and 2-Cys Prx subfamilies, respectively. Under different abiotic stresses(NaCl, PEG, CdCl2, low temperature and ABA), gene expression of ThPrx1 and ThPrx2 was investigated by using realtime quantitative RTPCR in roots and leaves. The results showed that ThPrx1 and ThPrx2 had different expression patterns under varied abiotic stresses. The mRNA levels of ThPrx1 in roots were upregulated under NaCl, PEG, and ABA treatments. ThPrx1 mRNA levels in both leaves and roots were downregulated under CdCl2 and low temperature stresses. The expression of ThPrx2 mRNA decreased during low temperature treatment, but the expression of ThPrx2 mRNA varied under other treatments. Our results demonstrate that ThPrx1 and ThPrx2 are two novel Prx genes, and they might play roles during different abiotic stress scenarios.
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Keywords:
- peroxiredoxins /
- Tamarix hispida /
- abiotic stress /
- gene expression
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