An efficient regeneration protocol had been established for plantlet regeneration from hypocotylinduced calli of Chrysanthemum lavandulifolium, which laid the foundation for genetic transformation of C. lavandulifolium. When long hypocotyls from the seeds that germinated in the dark for 7 days were cultured on the Murashige and Skoogs (MS) medium containing 1.0 mg/L 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 0.5 mg/L 6-benzyl aminopurine(6-BA) for 15 days, the calli inducing rate could reach the maximum of 86.66%. The calli were light yellow with almost uniform size. Following approximately 30 days of culture on the MS medium, adventitious buds were differentiated. The highest adventitious bud rate (25.50%) with an average of about 4.25 adventitious buds per explant was obtained from calli cultured on a MS medium without 6-BA. The rooting rate was 100% when the adventitious buds were cultured on the 1/2 MS medium supplemented with 0.1 mg/L naphthaleneacetic acid (NAA) for 15 days. After acclimatized to greenhouse conditions, normal growth and blossom C. lavandulifolium plants were obtained.