转TaLEA小黑杨矮化突变体的鉴定及侧翼序列分析
TaLEA overexpression in Populus simonii×P. nigra dwarf mutants and their identification and analysis of insertion site flanking sequences.
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摘要: 以转TaLEA 11个株系及野生型对照小黑杨为试材，通过生长性状、叶片解剖结构比较发现，在叶面积、气孔密度、叶片厚度等方面转基因XL11株系明显低于其他参试株系；角质层厚度、栅海比，气孔大小等方面 XL11株系显著高于其他参试株系。进而以XL11株系为材料，采用TAIL-PCR方法克隆插入位点的旁侧序列得到AP2/PthRAV（XM_0023114021）。荧光定量分析发现AP2/PthRAV的相对表达量在XL11株系中显著高于其他转基因株系及野生型平均值的7.78倍，初步认为该株系在生长及叶片解剖结构等性状的改变，可能与AP2/PthRAV上调表达有关。
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关键词:
- 转TaLEA小黑杨 /
- 矮化突变株 /
- AP2/PthRAV
Abstract: Eleven TaLEA overexpressed Populus simonii×P. nigra transgenic lines and wild-type control were studied for growth traits and leaf anatomy. The results showed that in regards to leaf area, stomatal density and leaf thickness, the XL11 lines were significantly lower than the other lines tested. On the contrary, XL11 lines epidermis, palisade and spongy tissue ratios and pore size were significantly higher than in the other lines. XL11 lines for the material,the TAIL-PCR method using cloned sequences flanking the insertion site and found AP2/PthRAV (XM_0023114021). Quantitative analysis of relative expression of the AP2/PthRAV lines in dwf1 was significantly higher than other transgenic lines, at 7-78 times of the average. A preliminary view of dwf1 lines in the change of growth and anatomical structure of leaf traits may be related to the upregulation of AP2/PthRAV genes. -
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