Abstract: To provide useful information for effective protection on nematode, the study focused on the investigation of species of parasitic nematode and their distribution in rhizoshphere soil of ornamental trees. The nematodes were isolated from the rhizoshpere of 40 ornamental trees in plantation nurseries at Qixia, Jiangning and Pukou of Nanjing, eastern China during October 2009 to July 2010. Based on the morphology and morphometric values, the nematodes were identified as Aphelenchus avenae, Aphelenchoides involutus, Xiphinema americanum and X. insigne. Moreover, 10 tree species were found to be the new host for X. americanum and 2 for X. insigne, respectively.