秋冬季自然降温过程中东北红豆杉幼苗的生理生化特性
Physiological and biochemical characteristics of Japanese yew seedlings as natural temperature falling in autumn and winter.
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摘要: 以4年生东北红豆杉实生苗为试验材料，分别测定了室外自然越冬幼苗以及初次室外越冬幼苗在秋冬季节自然降温过程中的多项生理生化指标，揭示了东北红豆杉幼苗适应低温条件的生理生化特性。结果表明：1）低温导致幼苗细胞膜透性增大。从9月至翌年1月，自然越冬幼苗的相对电导率由44.32%上升至12月的最高点52.98%，初次室外越冬幼苗由47.80%上升至12月的最高点62.12%。2）半致死温度下降。自然越冬幼苗和初次越冬幼苗的半致死温度的最低值分别为-37.5和-31.1 ℃。3）叶绿素含量下降。自然越冬幼苗和初次室外越冬幼苗翌年1月份的叶绿素含量相对9月份分别下降了24.7%和38.0%。4）可溶性蛋白质和游离脯氨酸含量呈先升高后下降趋势，可溶性糖含量逐月升高，2组幼苗间差异不显著。5）SOD和POD活性升高。自然越冬幼苗SOD和POD活性的最高值比9月升高了11.6%和17.2%，初次室外越冬幼苗SOD和POD活性的最高值比9月份升高了19.6%和5.9%。6）MDA含量逐月上升。翌年1月自然越冬幼苗和初次室外越冬幼苗的MDA含量较9月分别上升了26.8%和24.8%。初次室外越冬幼苗的多项指标都显示出其对低温的耐受力远低于自然越冬幼苗。Abstract: Japanese yew seedlings of four years old were chosen as experimental materials.Several physiological and biochemical indexes of two groups of Japanese yew seedlings were determined respectively as natural temperature falling in autumn and winter. The first group was growing under natural conditions,while the second group was growing indoor before the experiment. The results showed that: 1)the cell membrane permeability increased as temperature falling.The cell membrane permeability of the first group increased from 44.32% in September to the top point of 52.98% in December. While the second group increased from 47.80% to the top point of 62.12% in December. 2) The semilethal temperature of leaves decreased and the lowest semilethal temperature of two groups were -37.5 and -31.1 ℃.3)The contents of chlorophyll of two groups decreased by 24.7% and 380% respectively from September to January in the coming year.4)The contents of soluble protein and free praline increased firstly, and then decreased, the content of soluble sugar increased gradually, there was no significant difference between two groups. 5)The activities of superoxide dismutase（SOD）and peroxidase（POD）increased as well. The biggest value of SOD and POD of the first group increased by 11.6% and 17.2% respectively compared with that in September. The biggest value of SOD and POD of the second group increased by 19.6% and 5.9% respectively compared with that in September.6) MDA contents of both groups increased gradually from September to January in the coming year by 26.8% and 24.8% respectively. Furthermore, multitude indexes demonstrated that the seedlings in natural conditions had stronger tolerance to low temperature than the other group.
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期刊类型引用(2)
1. 杨秀琴, 梁亚男, 郑玉成, 苏晓. NMMO法棉纤维素薄膜的制备及性能分析. 塑料工业. 2016(12): 134-137 . 
百度学术
2. 马琨. 膜分离技术研究、企业生产现状及在水处理中的应用. 企业导报. 2014(01): 156-157 . 百度学术
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