枣和酸枣基因组大小测定
Negative regulation of gibberellin on root tip diameter.
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摘要: 本研究旨在找出最适合枣和酸枣的流式细胞术流程,进而测定它们的基因组大小。结果显示,在所对比的5 种裂解液中,WPB 最适合枣属植物细胞裂解。以毛果杨为内标,通过比较待测样品和内参G0/ G1 期峰,计算得出6 种枣属植物基因组大小。各枣品种的基因组大小有一定差异,平均基因组大小约为418.56 Mb,其中:‘冬枣爷的基 因组大小为(393.60 ±4.72)Mb,即C-值为(0.40 ±0.01)pg;‘鲁枣2 号爷的基因组大小为(428.00 ±3.61)Mb,即C- 值为(0.44 ±0.00)pg;‘金丝小枣爷的基因组大小为(424.00 ±5.81 )Mb,即C-值为(0.44 ±0.01)pg;‘孔府酥脆枣爷 的基因组大小为(429.60 ±5.03)Mb,即C-值为(0.44 ±0.01)pg;‘无核小枣爷的基因组大小为(413.60 ±7.07)Mb, 即C-值为(0.42 ±0.01 )pg;酸枣的基因组大小为(441.60 ±4.29)Mb,即C-值为(0.45 ±0.01)pg。优化的枣属植 物流式细胞术流程,为枣属植物的倍性鉴定奠定了基础Abstract: We conducted our study on tobacco plants for altered rates of biosynthesis and catabolism of GA constitutively or in specific tissues by transgenic approach and RNAi gene silencing technology. In the present study, PtGA20ox and PtGA2ox1 were over-expressed under the control of 35S promoter or root meristem-specific promoter (TobRB7) respectively and NtGA20ox was silenced. Root tips of transgenic plants and NtGA20ox RNAi lines were microscopic observation (n 100). It showed that transgenic plants compared with control had a very significant difference. This difference can be reversed by the exogenous application of GA3 and gibberellin synthesis inhibitor PAC. Moreover, similar phenotypes were obtained for root tip meristem-specific up-and down-regulation and constitutive regulation of endogenous gibberellin levels. The results provide evidence that GA negative regulates the root tip diameter through effects on the meristem
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Keywords:
- gibberellin /
- root tip diameter /
- radial growth /
- root meristem
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期刊类型引用(4)
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