摘要:
通过正交试验和酶活性测定法研究了落叶松毛虫6 龄幼虫乙酰胆碱酯酶(AChE)最佳反应体系及对3 种杀 虫剂的敏感性。结果表明:落叶松毛虫6 龄幼虫AChE 最佳反应条件为酶液质量浓度0郾2 g/ mL、底物浓度0郾8 mmol/ L、反应体系pH 7郾5、水浴温度35 益和反应时间5 min。敌敌畏、高效氯氟氰菊酯和阿维菌素对落叶松毛虫6 龄幼虫体外AChE 活性产生不同的抑制作用,且抑制效应与杀虫剂呈现明显的剂量鄄效应关系;其中敌敌畏抑制效 果最显著(IC50 =3.018 ·10 -4 mg/ mL),其次是高效氯氟氰菊酯(IC50 = 1.436 ·10 -2 mg/ mL),而阿维菌素对AChE 活性抑制效果最弱。
Abstract:
In this study, the optimal reaction system of acetylcholinesterase ( AChE) in 6th instar larval Dendrolimus superans was determined by orthogonal matrix method, then sensitivities of AChE to DDVP, lambda-cyhalothrin and avermectins were compared through enzyme assay. The results showed that the optimal conditions of AChE activity assay in 6th instar larval D. superans were 0.2 g/ mL of AChE concentration, 0.8 mmol/ L of substrate concentration, pH 7.5, 35 c of temperature and 5 minutes of reaction time. Moreover, the three pesticides, DDVP, lambda-cyhalothrin and avermectins, showed different inhibition to in vitro AChE activity in 6th instar larval D. superans. Relationship of inhibitory effect and pesticides was in a concentration way. Among three pesticides, DDVP showed the most significant inhibition to AChE activity in 6th instar larval D. superans (IC50 = 3.018 ∙10 -4 mg/ mL), following lambda-cyhalothrin(IC50 =1.436 ∙10 -2 mg/ mL), whereas avermectin was the weakest. These results will provide basal information for further studying the biochemical characteristics of AChE and toxicity mechanisms of pesticides.
百度学术
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