Abstract: This study was to explore the effects of precursors ( phenylalanine and cinnamate acid) and phytohormones (methyl jasmonate and salicylic acid) on the synthesis of pine polyphenols in the rind of Pinus karaiensis twigs. Possible mechanisms of their effects were also discussed. Our results showed that, phenylalanine and cinnamic acid as a precursor of the polyphenols synthesis, could increase the amount of pine polyphenols. The content of polyphenols increased by (11.74 ±1.09)mg/ g and (8.90 ±0.912) mg/ g,respectively when the best addition concentration of phenylalanine and cinnamic acid reached 400 and 100 μmol/ L. And phenylalanine and cinnamic acid had no synergy. On the other hand, phytohormone could effectively induce polyphenol synthesis. The content of pine polyphenols increased by (7.23 ±1.05) mg/ g and (14.68 ±1.04) mg/ g, respectively when the best addition concentration of methyl jasmonate acid and salicylic acid reached 60 and 80 μmol/ L. Methyl jasmonate acid and salicylic acid were antagonisitic to each other. So pine polyphenols in the bark of P. koraiensis can be promoted by the way of precursor feeding and hormonal induction.