响应面法优化樟子松树皮松多酚纯化工艺研究
Optimization of purification conditions of polyphenols from Pinus sylvestris var. mongolica using response surface methodology.
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摘要: 为了促进松多酚资源的综合利用,以樟子松树皮松多酚为原料,采用静态吸附和动态吸附实验从9 种大孔树 脂中筛选出适合纯化松多酚的大孔树脂,利用Box-Benhnken 设计对樟子松树皮松多酚初级纯化工艺条件进行优 化。结果表明:樟子松树皮松多酚初级纯化的最佳条件为上样液浓度2郾5 mg/ mL、上样液体积28 mL、洗脱速率10 mL/ min、洗脱剂乙醇浓度51.6%、径高比1∶27.3。优化后樟子松树皮松多酚纯度可达到69.23%。由此可知,AB鄄8 大孔树脂能较好地纯化富集樟子松松多酚。Abstract: We optimized the purification process of polyphenols from Pinus sylvetris var. mongolica bark to provide reference for its development. Static absorption-desorption and dynamic adscorption-desorption experiment were used to select the best one from nine different types of macroporous resin. Box-Benhnken design was used to optimize the optimum purification conditions. The optimized purification conditions of polyphenols from P. sylvetris var. mongolica were: sample solution concentration was 2.5 mg/ mL, 28 mL sample solution, eluting speed 10 mL/ min, the concentration of ethanol 51.6% and the ratio of diameter to height of resin column was 1∶27.3. Under the optimized purification conditions, purity of polyphenols was 69.23%. The results indicate that AB-8 macroporous resin has distinct effect on purification of polyphenols from P. sylvestris var. mongolica.
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Keywords:
- Pinus sylvestris var. mongolica /
- polyphenol /
- purify /
- response surface optimization
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期刊类型引用(4)
1. 王军民，王涛，杨莹，刘思远. 体外模拟消化对茯苓不同药用部位及代谢废料的抗氧化活性影响. 中国食用菌. 2021(11): 45-51 . 
百度学术
2. 赵玉红，翟亚楠，王振宇. 樟子松树皮多酚的成分分析和结构鉴定. 北京林业大学学报. 2016(01): 125-130 . 本站查看
3. 马艺丹，刘红，马思聪，闫瑞昕，廖小伟. 神秘果种子多酚大孔树脂纯化工艺研究. 食品与机械. 2016(02): 139-144 . 百度学术
4. 梁薇薇，王振宇，史锦硕. 真空冷冻干燥法制备松多酚微胶囊工艺研究. 安徽农业科学. 2014(36): 12956-12959 . 百度学术
其他类型引用(3)
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