摘要:
用乙醇提取红皮云杉中的抗氧化物质,分离纯化,并对各组分进行体外抗氧化能力研究。首先用不同浓度的
乙醇提取红皮云杉中的抗氧化物质,然后采用大孔树脂D101 纯化。收集不同组分,并对干物质及多酚等物质含量
较高部分做抗氧化能力的测定。通过对羟基自由基清除活性、DPPH 自由基清除活性、总还原力和抗脂质过氧化能
力的比较得出结论:PT40%鄄E 的羟基自由基清除活性最好,清除率可达97.998%,IC50 值为119.101g/mL;
PT20%-D 对DPPH的清除活性最好,清除率及IC50 分别为99.617% 和9.009g/mL;PT80%-D 总还原力最好,当
质量浓度为300g/mL 时吸光度值达2.209;PT40%-D 在抗脂质过氧化能力方面表现突出,抑制率为99.148%,
IC50为271.872g/mL。多酚、原花青素、黄酮类物质含量与抗氧化指标的相关性分析说明,多酚类物质在抗氧化中
起到了主要作用。
Abstract:
The objective of this study was to extract and purify antioxidant substances extracted from Picea
koraiensis by alcohol and evaluate their antioxidant activity in vitro. First, antioxidant substance was
extracted by different concentrations of ethanol and purified by macroporous resin D101. Then, different
samples were collected and the antioxidant activity of the components with the higher content of dry matter
and polyphenols was evaluated. Four antioxidant parameters (the capacity of scavenging hydroxyl and
DPPH radicals, total reducing power and lipid peroxidation inhibition effect) were measured. The results
indicated that PT40%-E showed the highest activity of scavenging hydroxyl radical (scavenging rate and
the value of IC50 were 97.998% and 119.101g/mL, respectively). PT20%-D showed the highest
activity of scavenging DPPH radical (scavenging rate and the value of IC50 were 99.617% and 9.009g/mL respectively). Then, PT80%-D showed higher ability of total reducing and the absorbance value was
2.209 when the concentration was 300g/mL. Finally, PT40%-D group showed the highest lipid
peroxidation inhibition effect (the inhibition ratio and the date of IC50 were 99.148% and 271.872g/
mL respectively ). The correlation analysis between the antioxidant activity and the content of
polyphenols, proanthocyanidins and flavonoids showed that the polyphenol was major contributor to
antioxidant properties.
百度学术
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