高级检索
    毛秀红, 朱士利, 李善文, 华辉, 田书勇, 仲伟国, 董玉峰, 安新民. 基于荧光SSR标记的毛白杨核心种质构建[J]. 北京林业大学学报, 2020, 42(7): 40-47. DOI: 10.12171/j.1000-1522.20190413
    引用本文: 毛秀红, 朱士利, 李善文, 华辉, 田书勇, 仲伟国, 董玉峰, 安新民. 基于荧光SSR标记的毛白杨核心种质构建[J]. 北京林业大学学报, 2020, 42(7): 40-47. DOI: 10.12171/j.1000-1522.20190413
    Mao Xiuhong, Zhu Shili, Li Shanwen, Hua Hui, Tian Shuyong, Zhong Weiguo, Dong Yufeng, An Xinmin. Core germplasm construction of Populus tomentosa based on the fluorescent SSR markers[J]. Journal of Beijing Forestry University, 2020, 42(7): 40-47. DOI: 10.12171/j.1000-1522.20190413
    Citation: Mao Xiuhong, Zhu Shili, Li Shanwen, Hua Hui, Tian Shuyong, Zhong Weiguo, Dong Yufeng, An Xinmin. Core germplasm construction of Populus tomentosa based on the fluorescent SSR markers[J]. Journal of Beijing Forestry University, 2020, 42(7): 40-47. DOI: 10.12171/j.1000-1522.20190413

    基于荧光SSR标记的毛白杨核心种质构建

    Core germplasm construction of Populus tomentosa based on the fluorescent SSR markers

    • 摘要:
        目的  研究毛白杨核心种质的取样策略,在进行相应的遗传分析基础上,构建核心种质,分析其遗传多样性,为毛白杨种质资源库建设、引种和新品种选育提供科学依据,亦为其他树种核心种质构建提供参考。
        方法  基于16对荧光SSR引物,利用毛细管电泳技术分析272份毛白杨和白杨杂种种质不同取样比例的遗传多样性参数。根据期望杂合度,计算每个样品对总体遗传多样性的贡献值,然后对所有样品根据贡献值从大到小进行排序。通过比较贡献率最高的前50%、45%、40%、35%、30%、25%、20%和15%取样比例获得的平均有效等位基因数(Ne)、平均Shannon信息指数(I)、平均期望杂合度(He)等,分析核心种质的代表性,确定其合适的取样比例。
        结果  随着取样比例的降低,Ne、I和He值均在升高,均大于原始种质相应数值,而且He值均大于0.5,表明具有丰富的遗传多样性,而原始种质的He值小于0.5。按照25%取样比例,得到前68名种质,其中包含18份杂种种质以及所有省份选出的部分优异种质。所得到的Ne、I和He分别是2.761、1.094和0.539,均大于原始种质的相应值2.075、0.825和0.432。t检测结果表明,核心种质与原始种质的遗传多样性无显著差异,表明这68份种质在遗传多样性方面具有可靠的代表性,可以作为核心种质。北京的种质与河北的种质遗传一致度最高,为0.997;与山西次之,为0.990。
        结论  毛白杨核心种质的最佳取样比例是25%,最佳取样范围为20% ~ 40%,如果种质资源数目较大,可以适当降低至15%,如果基数较小,可以升高至45%。He、Ne、I等均表明这些核心种质具有丰富的遗传多样性。杂种种质遗传变异丰富,聚合了亲本的优良等位基因,首次从分子水平证明了杂种种质是毛白杨遗传改良的重要育种资源。建议相关部门或者育种者要高度重视白杨杂种种质的收集、保存和再利用。

       

      Abstract:
        Objective  To study the sampling proportion and to determine the sampling strategy on the basis of corresponding genetic analysis, analyzing the genetic diversity and constructing the core germplasm of P. tomentosa can provide molecular foundation for the introduction, genetic breeding, collection and preservation of Leuce germplasm resources. It also can provide reference for the core germplasm construction of other tree species.
        Method  Based on 16 pairs of fluorescent SSR primers, the genetic diversity of 272 accessions of P. tomentosa and poplar hybrid was analyzed by capillary electrophoresis. Based on expected heterozygosity data, all samples were ranked according to their contribution to the overall genetic diversity. The sampling ratios from top to low were 50%, 45%, 40%, 35%, 30%, 25%, 20% and 15%. The representativeness of core germplasm was analyzed by comparing the average effective allele number Ne, the average Shannon information index I and the average expected heterozygosity He, et al. Then the appropriate sampling proportion was determined.
        Result  With the decrease of sampling proportion, the values of Ne, I and He were all increasing, which were all greater than the corresponding values of the original germplasm. The values of He were all greater than 0.5, indicating abundant genetic diversity, while the values of the original germplasms were less than 0.5. According to 25% sampling ratio, the first 68 germplasms were obtained, including 18 hybrid germplasms and some excellent germplasms selected by all provinces. The Ne, I and He values were 2.761, 1.094 and 0.539, respectively, which were all higher than the corresponding values of the original germplasm 2.075, 0.825 and 0.432. It was showed by the t-test that there was no significant difference between the genetic diversity of the core germplasm and the original germplasm resources, indicating that these 68 germplasms have a very reliable representation in terms of genetic diversity and can be used as the core germplasm. The genetic consistency between Beijing germplasm and Hebei germplasm was 0.997, followed by Shanxi of 0.990.
        Conclusion  The best sampling proportion of core germplasm of P. tomentosa is 25%, and the best sampling range is 20%−40%. If the number of germplasm resources is large, it can be reduced to 15%; if the base is small, it can be increased to 45%. He, Ne, I, etc. all indicate that the above core germplasm has abundant genetic diversity. The genetic variation of hybrid germplasm is rich, which aggregates the good alleles of parents. It is proved at the molecular level that hybrid germplasm is an important breeding resource for genetic improvement of P. tomentosa. We suggest that relevant departments or breeders should attach great importance to the collection, preservation and reuse of poplar hybrid germplasm.

       

    /

    返回文章
    返回